PrecisION hKv1.6-CHO Recombinant Cell Line

PrecisION hKv1.6-CHO Recombinant Cell Line

Upstate (Millipore)

2 mL

Recombinant CHO-K1 cell line expressing human Kv1.6.

Format:
2 x 1 ml aliquots containing 3.04 x 10⁶cells/ml in 77.5% DMSO at passage 9.

Kv1.6 is expressed in the brain (astrocytes, oligodendrocytes), colon, germ cells, heart, lung and pulmonary artery smooth muscle cells. This ion channel is involved with the maintenance of the resting membrane potential and therefore regulates the activity of excitable cells.

CHO-K1 cells expressing hKv1.6 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 60 mV was 1740 ± 314 pA (n=5). The threshold for current activation was around -50 mV and exhibited very slow C-type inactivation at depolarized potentials. These properties are characteristic of Kv1.6 currents described in the literature. TEA (5 mM) significantly blocked the current (68 ± 3%, n=4) which is consistent with previous reports describing Kv1.6 sensitivity to TEA. Margatoxin (1-10 nM) also blocked current responses as expected for selective expression of Kv1.6 channels. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 43 passages: 86% of cells expressed Kv1.6 currents of >500 pA at passage 43.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class. The coding region of this gene is intronless, and the gene is clustered with genes KCNA1 and KCNA5 on chromosome 12.

FUNCTION: SwissProt: P17658 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 529 amino acids; 58729 Da
SUBUNIT: Heterotetramer of potassium channel proteins (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P17658 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.

Human

Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species

CHO

Ion Channel Cell Lines

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial

Upstate

Chinese Hamster Ovarian K-1 cells (CHO K-1)

2 x 1 ml aliquots

hKv1.6

P17658

Potassium

2 x 1 ml aliquots

• KCNA6
• Kv1.6
• KV1.6
• HBK2

Ion Channels

PrecisION hKv1.6-CHO Recombinant Cell Line

NM_002235.2

• KCNA6
• Kv1.6
• KV1.6
• HBK2