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PrecisION hKv1.7-CHO Recombinant Cell Line
Description:
PrecisION hKv1.7-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing the human voltage-gated potassium channel Kv1.7.
Background Information:
Kv1.7 is a voltage-gated, delayed rectifier, potassium channel. It is expressed in the heart, skeletal muscle and pulmonary arteries. As this channel has properties similar to the ultra-rapidly activating (IKur) current of the heart, it is potentially a candidate channel for involvement in human cardiac disorders.
Application Notes:
CHO-K1 cells expressing hKv1.7 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 60 mV was 2.69 ± 0.8 nA (n=4). The threshold for current activation was around -20 mV and exhibited a very slow C-type inactivation at depolarized potentials. TEA (50 mM) significantly blocked the current (56-61%) which is consistent with previous reports describing Kv1.7 sensitivity to TEA. 4-AP (100 μM) also blocked the current (82%) as expected for the selective expression of Kv1.7 channels. Similarly ShK (10 mM/50 mM) blocked the current by 17.5%/30.6% respectively. Functional expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 44 passages: 94.2% of cells expressed Kv1.7 currents of >500 pA at passage 44.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. The gene is expressed preferentially in skeletal muscle, heart and kidney. It is a candidate gene for inherited cardiac disorders.
UniProt Summary:
FUNCTION: SwissProt: Q96RP8 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient (By similarity).
SIZE: 456 amino acids; 50559 Da
SUBUNIT: Heterotetramer of potassium channel proteins (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart and kidney.
DOMAIN: SwissProt: Q96RP8 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments (By similarity). & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position (By similarity).
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
SIZE: 456 amino acids; 50559 Da
SUBUNIT: Heterotetramer of potassium channel proteins (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart and kidney.
DOMAIN: SwissProt: Q96RP8 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments (By similarity). & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position (By similarity).
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv1.7
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNA7
- KV1.7
- K()1.7
- HAK6
- Kv1.7
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv1.7-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNA7
- KV1.7
- K()1.7
- HAK6
- Kv1.7