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PrecisION hKv2.1-CHO Recombinant Cell Line
Description:
PrecisION hKv2.1-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv2.1.
Format:
2 x 1 ml aliquots containing 2.30 x 106cells/ml in 10% DMSO at passage 12.
Format:
2 x 1 ml aliquots containing 2.30 x 106cells/ml in 10% DMSO at passage 12.
Background Information:
Kv2.1 is expressed in the brain (cell bodies and dendrites of neurons but not axons, as well as Schwann cells), atria, ventricle, skeletal muscle, cochlea, retina, lung and pulmonary arteries. It is involved in maintaining membrane potential and modulating electrical excitability of both neurons and muscle cells. It is thought that Kv2.1 has a role in the muscle tone of the pulmonary system, as the expression Kv2.1 in pulmonary smooth muscle has been found to be reduced in chronic hypoxic pulmonary hypertension.
Application Notes:
CHO-K1 cells expressing hKv2.1 were characterised in terms of their biophysical and pharmacological properties using whole-cell patch clamp techniques. The mean current amplitude at 60 mV was 5.63 ± 0.86 nA (n=11). The threshold for current activation was between -20 and -30 mV. These properties are characteristic of hKv2.1 currents described in the literature. The cell-line is shown to be expressing hKv2.1 current since it is blocked by low mM concentrations of the potassium channel blockers 4-aminopyridine (10 mM; 73.9 ± 1.7 %, n=3) and tetraethylammonium (10 mM; 76.0 ± 0.1%, n=3-4). Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 26 passages: 76% of cells expressed outward current >500 pA at passage 26 (n=117). The mean current amplitude of these cells was 2420 ± 120 pA.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier potassium channel and its activity is modulated by some other family members.
UniProt Summary:
FUNCTION: SwissProt: Q14721 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
SIZE: 858 amino acids; 95878 Da
SUBUNIT: Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q14721 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. & The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
PTM: Phosphorylated on serine residues in the C-terminal cytoplasmic tail (By similarity).
SIMILARITY: Belongs to the potassium channel family. B (Shab) subfamily.
SIZE: 858 amino acids; 95878 Da
SUBUNIT: Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q14721 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. & The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
PTM: Phosphorylated on serine residues in the C-terminal cytoplasmic tail (By similarity).
SIMILARITY: Belongs to the potassium channel family. B (Shab) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv2.1
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNB1
- KV2.1
- h-DRK1
- DRK1
- Kv2.1
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv2.1-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNB1
- KV2.1
- h-DRK1
- DRK1
- Kv2.1