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PrecisION hNav1.2-CHO Recombinant Cell Line
Description:
PrecisION hNav1.2-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Nav1.2 (type II, voltage-gated sodium channel, alpha subunit (SCN2A)).
Format:
2 x 1 ml aliquots containing 1.20 x 106cells/ml in 7.5% 10% DMSO at passage 6.
Format:
2 x 1 ml aliquots containing 1.20 x 106cells/ml in 7.5% 10% DMSO at passage 6.
Background Information:
The voltage gated sodium channel isoform Nav1.2 is involved with axon potential initiation and conduction. Nav1.2 is the target of antiepileptic drugs and the probable target causing side effects of centrally active local anaesthetics. It is detected in relative abundance on myelin-deficient areas on axons in the central and peripheral nervous systems. Demyelinated axons expressing Nav1.2 are much less susceptible to axonal degeneration. The action of a drug on Nav1.2 sodium channels may interfere with the fine balance between axon growth and nerve impulse transmission.
Application Notes:
CHO-K1 cells expressing hNav1.2 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques, IonWorks™ HT and IonWorks Quattro™. The threshold for current activation was around -40 mV and exhibited complete inactivation at all test voltages (-90 mV to 100 mV) during the 20 ms step. The voltage for half steady state activation (V1/2) was approximately -65 mV. These properties are characteristic of Nav1.2 currents described in the literature. TTX blocked the current in a dose-dependent manner (IC50 value =6.5 nM in whole-cell patch-clamp electrophysiology and =5 nM in IonWorks Quattro™). Using an 8-pulse voltage protocol, tetracaine dose-dependently blocked hNav1.2 currents with IC50 values of 23 μM and 0.7 μM for the 1st and 8th pulse respectively. Functional channel expression over time was monitored using IonWorksTM HT. Channel expression is robust over at least 25 passages: 93% of cells expressed hNav1.2 currents of >500 pA at passage 23.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. It is heterogeneously expressed in the brain, and mutations in this gene have been linked to several seizure disorders. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
UniProt Summary:
FUNCTION: SwissProt: Q99250 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
SIZE: 2005 amino acids; 227975 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q99250 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis (By similarity).
DISEASE: SwissProt: Q99250 # Defects in SCN2A are a cause of generalized epilepsy with febrile seizures plus (GEFS+) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN2A are a cause of benign familial neonatal- infantile seizures (BFNIS) [MIM:607745]. BFNIS is an autosomal dominant epilepsy syndromes well recognized in the first year of life. It may be associated with paroxysmal dyskinesias in later childhood and can also occur as a non-familial form.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
SIZE: 2005 amino acids; 227975 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q99250 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis (By similarity).
DISEASE: SwissProt: Q99250 # Defects in SCN2A are a cause of generalized epilepsy with febrile seizures plus (GEFS+) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN2A are a cause of benign familial neonatal- infantile seizures (BFNIS) [MIM:607745]. BFNIS is an autosomal dominant epilepsy syndromes well recognized in the first year of life. It may be associated with paroxysmal dyskinesias in later childhood and can also occur as a non-familial form.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hNav1.2
UniProt Number:
Target Sub-Family:
Sodium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- SCN2A
- NAC2
- HBSCI
- SCN2A2
- Na(v)1.2
- HBA
- Nav1.2
- HBSCII
- SCN2A1
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hNav1.2-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- SCN2A
- NAC2
- HBSCI
- SCN2A2
- Na(v)1.2
- HBA
- Nav1.2
- HBSCII
- SCN2A1