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PrecisION hKv4.2/hKChIP2-CHO Recombinant Cell Line
Description:
PrecisION hKv4.2/hKChIP2-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv4.2 and human KChIP2.
Format:
2 x 1 ml aliquots containing 2.10 x 106cells/ml in 7.5% DMSO at passage 9.
Format:
2 x 1 ml aliquots containing 2.10 x 106cells/ml in 7.5% DMSO at passage 9.
Application Notes:
CHO-K1 cells expressing hKv4.2 and hkChIP2 were characterized in terms of their pharmacological and biophysical properties using IonWorks™ HT. The presence of both subunits is stronly supported by the current's rapid activation and inactivation kinetics and by theh apparent rapid recovery from inactivation (>20 ms). The hKv4.2/hKChIP current was inhibitors by the blockers arachidonic acid bepridril, quinidine, and nicardipine with IC50 calues of 2.82 μM, 11.8 μM, 16.1 μM, and 3.78 μM respectively. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 28 passages with >83% of cells forming seals and of these >86% expressing hKv4.2/hKCHIP2 currents of >500pA (n=1912).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels.
UniProt Summary:
FUNCTION: SwissProt: Q9NZV8 # Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
SIZE: 630 amino acids; 70537 Da
SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND3. Interacts with DPP6, DLG4 and FREQ (By similarity). Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Interacts with FLNA, FLNC and DPP10.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
TISSUE SPECIFICITY: Highly expressed throughout the brain. Expression is very low or absent in other tissues.DOMAIN:SwissProt: Q9NZV8 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Phosphorylated on serine and threonine residues (By similarity).
SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily.
SIZE: 630 amino acids; 70537 Da
SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND3. Interacts with DPP6, DLG4 and FREQ (By similarity). Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Interacts with FLNA, FLNC and DPP10.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
TISSUE SPECIFICITY: Highly expressed throughout the brain. Expression is very low or absent in other tissues.DOMAIN:SwissProt: Q9NZV8 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Phosphorylated on serine and threonine residues (By similarity).
SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv4.2/hKChIP2
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCND2
- RK5
- Kv4.2
- KV4.2
- MGC119703
- KIAA1044
- MGC119702
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv4.2/hKChIP2-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCND2
- RK5
- Kv4.2
- KV4.2
- MGC119703
- KIAA1044
- MGC119702