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PrecisION hKv4.3/hKChIP1-CHO Recombinant Cell Line
Description:
PrecisION hKv4.3/hKChIP1-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv4.3 and human KChIP1
Format:
2 x 1 ml aliquots containing 1.04 x 106cells/ml in 10% DMSO at passage 8.
Format:
2 x 1 ml aliquots containing 1.04 x 106cells/ml in 10% DMSO at passage 8.
Background Information:
Rapidlly inactivating voltage sensitive potassium channels are found both in neurons (A-current) and cardiac myocytes (Ito) where they are responsible of the ‘notch’ in phase 1 of the cardiac action potential. The channels underlying Ito in humans are principally Kv4.3 and the regional differences in expression are thought to be governed by levels of the accessory protein KChIP2. Due to their critical role in controlling the trajectory of the action potential to differing degrees in various regions of the heart, blockade of this channel could lead to increased arrhythmic risk. In cortical, hippocampal, and striatal interneurons, KChIP1 is frequently co localized with Kv4.3. This complex of human Kv4.3 and KChIP1 represents a physiologically relevant cell line.
Application Notes:
CHO-K1 cells expressing hKv4.3 and hKChIP1 were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques and IonWorks™ HT. Co-expression of both subunits is strongly supported by the increase in the mean peak currents with respect to expression of Kv4.3 alone (from 9 to 22 nA as recorded using whole-cell patch clamp techniques and from 2.5 to 4.2 nA as measured with IonWorks™ HT). In addition cells expressing both subunits displayed a more rapid recovery from inactivation (141 ms) with respect to cells expressing just hKv4.3 (1319 ms). The hKv4.3/hKChIP1 current was inhibited by the antagonists, quinidine and flecainide, with IC50 values of 20 µM and 58 µM respectively.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Protein Target:
hKv4.3/hKChIP1
Presentation:
2 x 1 ml aliquots
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv4.3/hKChIP1-CHO Recombinant Cell Line