PrecisION hKir2.1-HEK Recombinant Cell Line

PrecisION hKir2.1-HEK Recombinant Cell Line

Upstate (Millipore)

2 mL

Recombinant HEK293 cell line expressing the human Kir2.1 [inwardly-rectifying voltage-gated potassium channel, subfamily J, member 2 (KCNJ2), Accession Number NM_00891].

Format: 2 x 1 ml aliquots containing 1.12 x 106 cells/ml in 10% DMSO at passage 15

Kir2.1 has a fundamental role in controlling the resting potential of cardiac myocytes and shaping the cardiac action potential. It is therefore likely that drugs affecting this channel will have an effect on cardiac excitability. Mutations in the KCNJ2 gene that encodes Kir2.1, reduce expression of the channel and cause a form of long QT syndrome (Andersen Syndrome, LQT7) that predisposes individuals to ventricular arrhythmias.Recognition that the dysfunction of this channel can lead to long QT has lead to it’s inclusion into the cardiac panel.

The electrophysiological and pharmacological properties of the cell line were examined using conventional whole-cell recording techniques. The current voltage relationship and activation/inactivation kinetics were similar to previously reported data for hNav1.4 currents. The currents also exhibited state-dependent block using lidocaine and were potently blocked by μ-conotoxin GIIIA, a selective blocker of muscle sodium channels. Functional channel expression over time was monitored using IonWorks™ HT. At least 80% of cells (range 80-100%) expressed hNav1.4 currents over 500 pA for 40 passages.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.

FUNCTION: SwissProt: P63252 # Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
SIZE: 427 amino acids; 48288 Da
SUBUNIT: Homomultimeric and heteromultimeric association with Kir2.3, resulting in an enhanced G-protein-induced current. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
DISEASE: SwissProt: P63252 # Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features. & Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
SIMILARITY: SwissProt: P63252 ## Belongs to the inward rectifier-type potassium channel family.

Human

Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species

HEK

Ion Channel Cell Lines

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial

Upstate

293 cell line

2 x 1 ml aliquots

hKir2.1

P63252

Potassium

2 x 1 ml aliquots

• KCNJ2
• IRK1
• LQT7
• HIRK1
• Kir2.1
• HHBIRK1
• SQT3
• HHIRK1
• KIR2.1

Ion Channels

PrecisION hKir2.1-HEK Recombinant Cell Line

NM_000891.2

• KCNJ2
• IRK1
• LQT7
• HIRK1
• Kir2.1
• HHBIRK1
• SQT3
• HHIRK1
• KIR2.1