Content Loading...
Content Loading...
PrecisION hHCN1-HEK Recombinant Cell Line
Description:
PrecisION hHCN1-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the human hyperpolarization-activated, cyclic nucleotide-gated potassium channel 1 (HCN1).
Format:
2 x 1 ml aliquots containing 1.20 x 106cells/ml in 7.5% DMSO at passage 15.
Format:
2 x 1 ml aliquots containing 1.20 x 106cells/ml in 7.5% DMSO at passage 15.
Background Information:
HCN channels contribute to rhythmic spontaneous electrical activity in the heart and CNS. Interference with HCN1 activity can lead to various forms of arrhythmias. The spontaneous neuronal activity of HCN channels have also been implicated in the development and maintenance of a wide range of pain states and an inherited loss of HCN1 channel expression is involved in generalized idiopathic epileptic seizures
Application Notes:
HEK293 cells expressing hHCN1 were characterised in terms of their biophysical and pharmacological properties using whole-cell patch clamp techniques and IonWorksTM HT electrophysiology. The biophysical properties are typical of hHCN1 both in terms of V½ of activation (-86 ± 3 mV, n=11) and the time course of activation (e.g. 349 ± 130 ms at -110 mV and 104 ± 32 ms at -140 mV, n=6). Under whole-cell recording conditions the mean current amplitude was 2.7 ± 0.5 nA at -130 mV (n =10) and reversal potential measurements indicated permeability to both Na+ and K+ (PNa/PK around 0.2) typical of HCN channels.
Currents were dose-dependently inhibited by the selective HCN blocker ZD7288 (IC50 value of 44 µM) and the inorganic cation Caesium (Cs+, IC50 value of 114 µM) in line with reported literature values. The currents were also insensitive (<10% inhibition) to the tyrosine kinase inhibitor genistein (100 µM) although the same concentration markedly inhibited hHCN2 currents (approximately 66% inhibition, Millipore PrecisIONTM HCN2-HEK293 recombinant cell line, CYL3041). These differences in sensitivity have been noted by others and support the notion that this cell line selectively expresses hHCN1 channels. Channel expression, monitored using IonWorksTM HT, is robust over at least 40 passages. For example 76% of cells expressed outward current >500 pA at passage 40 (n=123) with a mean current amplitude of 1.41 nA.
Currents were dose-dependently inhibited by the selective HCN blocker ZD7288 (IC50 value of 44 µM) and the inorganic cation Caesium (Cs+, IC50 value of 114 µM) in line with reported literature values. The currents were also insensitive (<10% inhibition) to the tyrosine kinase inhibitor genistein (100 µM) although the same concentration markedly inhibited hHCN2 currents (approximately 66% inhibition, Millipore PrecisIONTM HCN2-HEK293 recombinant cell line, CYL3041). These differences in sensitivity have been noted by others and support the notion that this cell line selectively expresses hHCN1 channels. Channel expression, monitored using IonWorksTM HT, is robust over at least 40 passages. For example 76% of cells expressed outward current >500 pA at passage 40 (n=123) with a mean current amplitude of 1.41 nA.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Protein Target:
hHCN1
Presentation:
2 x 1 ml aliquots
Target Sub-Family:
Hyperpolarized
Packaging:
2 x 1 ml aliquots
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hHCN1-HEK Recombinant Cell Line