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PrecisION hKv3.1-CHO Recombinant Cell Line
Description:
PrecisION hKv3.1-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv3.1 potassium ion channel
Background Information:
Kv3.1 mRNA is abundantly expressed in auditory brain stem neurons that are able to spike at high frequencies. It has a high threshold of activation and rapid activation/deactivation kinetics (Wang et al., 1998). Evidence for functional Kv3.1 activity in such neurons can be derived from the rapidly activating and deactivating potassium currents seen here, that activate at a high-voltage threshold similar to that of heterologously expressed Kv3.1 (Gan and Kaczmareck, 1998). There are indications that the phosphorylation state in rat Kv3.1 may play a part in the ability of auditory neurons to adjust to the ambient acoustic environment (Song et al., 2005). It is thought that Khv3.1 subunits are likely to form homomultimers in lymphocytes and therefore could be a target for the development of novel immunosuppresants. (Grissmer et al., 1994).
Application Notes:
CHO-K1 cells expressing hKv3.1 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques and IonWorks™ HT. Using whole-cell patch clamp techniques the peak I/V relationship was linear and had a high threshold for current activation (-20 mV), typical of hKv3.1 currents. Riluzole and TEA have been shown to block hKv3.1 currents in the micromolar range. For example, application of TEA (200 µM) or riluzole (300 µM) blocked currents within a similar concentration range; 39.6 ± 3.1 % inhibition (peak current, n=5) and 39.9 ± 6.0 % inhibition (end current, n=5) respectively. Functional channel expression over time was monitored using IonWorksTM HT. Channel expression is robust over at least 31 passages. The mean peak hKv3.1 outward current amplitude on stepping to +30 mV from a holding potential of -80 mV was consistently between 3–4 nA. Furthermore, of the cells that sealed, the percent of cells expressing hKv3.1 currents (i.e. currents >500 pA) was consistently between 98–100 %.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Protein Target:
hKv3.1
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
KCNC1
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv3.1-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
KCNC1