PrecisION hKv3.3-CHO Recombinant Cell Line

PrecisION hKv3.3-CHO Recombinant Cell Line

Upstate (Millipore)

2 mL

Recombinant CHO-K1 cell line expressing the human voltage-gated potassium channel Kv3.3.

Format: 2 x 1ml vials (1.80 x 10⁶ cells/ml) of hKv3.3-CHO K1 at passage 11

Kv3.3 are voltage-gated, fast inactivating Shaw channels which play an important role in regulating neuronal output by modulating the delay and frequency of high frequency neuronal firing. They are highly expressed in areas of the brain involved with motor control such as the cerebellum. They have also been shown be expressed at the mammalian neuromuscular junction where they are thought to regulate transmitter release.

CHO-K1 cells expressing hKv3.3 were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques and IonWorksTM HT. Using whole-cell patch clamp techniques the threshold of current activation was found to occur when the membrane potential was depolarised to potentials more positive than -20 mV, with the current inactivating at potentials greater than 0 mV. The mean current at +40 mV was 11.69 ± 1.06 nA (n = 5). The values of V½ of activation and inactivation were approximately 10 mV and –19 mV respectively, in line with published data. Currents were inhibited by µM concentrations of the known potassium channel blockers TEA and 4-AP. The currents obtained with IonWorksTM HT were typical of homomeric hKv3.3 currents since they had an initial fast activating component followed by a slowly inactivating component. Functional channel expression over time was monitored using IonWorksTM HT. Channel expression is robust over at least 37 passages. At passage 37, 95% of cells sealed with a resistance greater than 50 MOhm. Of these, 100% expressed hKv3.3 currents greater than 500 pA with a mean current amplitude of 4.80 ± 0.10 nA (n=183).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes.

FUNCTION: SwissProt: Q14003 # This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 757 amino acids; 80520 Da
SUBUNIT: Heterotetramer of potassium channel proteins (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q14003 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. & The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
DISEASE: SwissProt: Q14003 # Defects in KCNC3 are the cause of spinocerebellar ataxia 13 (SCA13) [MIM:605259]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA13 is a slow progressing form. Age at onset is variable, ranging from childhood to late adulthood. Mental retardation can be present in some patients.
SIMILARITY: Belongs to the potassium channel family. C (Shaw) subfamily.

Human

Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species

CHO

Ion Channel Cell Lines

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial

Upstate

Chinese Hamster Ovarian K-1 cells (CHO K-1)

2 x 1 ml aliquots

hKv3.3

Q14003

Potassium

2 x 1 ml aliquots

• KCNC3
• SCA13
• K()3.3
• Kv3.3
• KV3.3
• KSHIIID

Ion Channels

PrecisION hKv3.3-CHO Recombinant Cell Line

NM_004977.2

• KCNC3
• SCA13
• K()3.3
• Kv3.3
• KV3.3
• KSHIIID