PrecisION rNav1.8-ND7 Recombinant Cell Line

PrecisION rNav1.8-ND7 Recombinant Cell Line

Upstate (Millipore)

2 mL

Recombinant ND7-23 (rat DRG/mouse neuroblastoma hybrid) cell line expressing the rat Nav1.8 (type 10 voltage-gated sodium channel, alpha polypeptide (Scn10a)).

Format:
2 x 1 ml aliquots containing 1.08 x 10⁶cells/ml in 10% DMSO at passage 7.

Nav1.8 subtype selective blockers are predicted to be effective in the treatment of neuropathic pain and to be associated with fewer adverse effects than currently available therapies. Drugs for the clinical treatment of neuropathic pain can interact with other members of the voltage-gated sodium channel (Nav1) family due to their close sequence homology.

ND7-23 cells expressing rNav1.8 were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The cell line was shown to be expressing rNav1.8 currents since it was resistant to TTX (100 nM) but blocked by tetracaine in the micromolar range. The currents also had biophysical properties similar to published data where Nav1.8 has been expressed in recombinant systems. For example they were activated at potentials more positive to -45 mV, the peak of I/V relationship was 0/+10 mV, V½ of activation was –9 mV and V½ of inactivation was around –50 mV. Channel expression, monitored using IonWorksTM HT, is stable over at least 70 passages. Typically, for a given passage, over 80% of the cells express peak inward currents greater than 200 pA with a mean amplitude of between 500-600 pA. This cell line is also suitable for voltage sensitive probe assays. Using such an assay, the sodium channel opener deltamethrin induced Na+-dependent membrane potential depolarisations with an EC50 of approximately 1 µM that were blocked by micromolar concentrations of tetracaine.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Voltage-gated sodium channels are integral membrane glycoproteins that are responsible for the initial rising phase of action in most excitable cells. They are composed of a large alpha subunit that may be associated with one or more smaller beta subunits. Sodium channels can be differentiated by their primary structure, kinetics, and relative sensitivity to the neurotoxin tetrodotoxin (TTX). Sodium channels, particularly those with TTX-resistant currents, have been found to accumulate in the region of peripheral nerve injury and may be important in chronic pain. SCN10A encodes a TTX-resistant channel that is restricted to the peripheral sensory nervous system (Rabert et al., 1998 [PubMed 9839820]).[supplied by OMIM]

FUNCTION: SwissProt: Q9Y5Y9 # This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms (By similarity).
SIZE: 1956 amino acids; 220566 Da
SUBUNIT: The voltage-resistant sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more associated auxiliary subunits SCN1B, SCN2B and SCN3B. Found in a number of complexes with PRX, TCTEL1 and PDZK3. Interacts with proteins such as FSTL1, PRX, TCTEL1, PDZK3, S100A10 and many others (By similarity). Interacts with NEDD4 and NEDD4L.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity). Note=It can be translocated to the extracellular membrane through association with S100A10 (By similarity).TISSUE SPECIFICITY: Expressed in the dorsal root ganglia and sciatic nerve.
DOMAIN: SwissProt: Q9Y5Y9 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: Ubiquitinated by NEDD4L; which promotes its endocytosis (By similarity).
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.

Rat

Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species

Rat DRG/Mouse Neuroblastoma Hybrid

Ion Channel Cell Lines

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial

Upstate

2 x 1 ml aliquots

rNav1.8

Q9Y5Y9

Sodium

2 x 1 ml aliquots

• SCN10A
• PN3
• Nav1.8
• SNS
• hPN3

Ion Channels

PrecisION rNav1.8-ND7 Recombinant Cell Line

NM_006514.2

• SCN10A
• PN3
• Nav1.8
• SNS
• hPN3