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PrecisION hnAChR α1/β1/δ/ε-HEK Recombinant Cell Line
Description:
PrecisION hnAChR α1/β1/δ/ε-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the four subunits α1, β1, δ and ε of the human nicotinic acetylcholine receptor type I.
Format:
2 x 1 ml aliquots containing 3.34 x 106cells/ml in 7.5% DMSO at passage 11.
Format:
2 x 1 ml aliquots containing 3.34 x 106cells/ml in 7.5% DMSO at passage 11.
Background Information:
The α1/β1/δ/ε receptor is the major nicotinic receptor found in adult mammalian muscle. These muscle nACh receptors are involved in the fast synaptic transmission at the skeletal-muscular junction and their activation causes muscular contraction. This cell line would be useful for safety counter screening compounds for any undesirable action on this nicotinic receptor subtype.
Application Notes:
HEK293 cells expressing hnAChR α1/β1/δ/ε were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques and FLIPR calcium assay. Using whole-cell patch clamp techniques, the mean peak current in response to 100 µM ACh was 1418 pA. The mean pEC50 value for the ACh-elicited current was 4.9. D-tubocurarine (10 µM) blocked the 100 µM ACh-elicited response by a mean of 94%. In FLIPR calcium assays the agonists epibatidine, succinyldicholine and nicotine had pEC50 values of 6.3, 5.5 and 4.4 respectively. The antagonists pancuronium, D-tubocurarine and mecamylamine blocked the 60 µM succinyldicholine agonist response with a pIC50 values of 6.4, 6.3 and 4.5 respectively. Functional channel expression over time was monitored using FLIPR calcium assays. Channel expression is robust over at least 30 passages. At passage 30 the fluorescence signal window was greater than 11,000 with a Z’ value of approximately 0.7.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
UniProt Summary:
FUNCTION: SwissProt: Q07001 # After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
SIZE: 517 amino acids; 58895 Da
SUBUNIT: Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: Q07001 # Defects in CHRND are a cause of slow-channel congenital myasthenic syndrome (SCCMS) [MIM:601462, 254200]. Congenital myasthenic syndromes (CMS) are inherited disorders of neuromuscular transmission that stem from mutations in presynaptic, synaptic, or postsynaptic proteins. Postsynaptic disorders result from mutations in proteins forming the subunits of the muscle acetylcholine receptor (AChR). The kinetic abnormalities of AChR result in either prolonged ion channel activations that underlie slow-channel myasthenic syndromes (SCCMS) or abbreviated channel activations that underlie the abnormally rapid decay of endplate currents in fast-channel syndromes (FCCMS). A third disorder associated with postsynaptic CMS is called CMS type Id (CMS1d), and could also result from mutations in the proteins forming the subunits of the muscle AChR. Mutations underlying SCCMS cause a gain of function and usually show dominant inheritance. & Defects in CHRND are a cause of fast-channel congenital myasthenic syndrome (FCCMS) [MIM:608930]. Mutations underlying FCCMS cause a loss of function and show recessive inheritance.
SIMILARITY: SwissProt: Q07001 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
SIZE: 517 amino acids; 58895 Da
SUBUNIT: Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: Q07001 # Defects in CHRND are a cause of slow-channel congenital myasthenic syndrome (SCCMS) [MIM:601462, 254200]. Congenital myasthenic syndromes (CMS) are inherited disorders of neuromuscular transmission that stem from mutations in presynaptic, synaptic, or postsynaptic proteins. Postsynaptic disorders result from mutations in proteins forming the subunits of the muscle acetylcholine receptor (AChR). The kinetic abnormalities of AChR result in either prolonged ion channel activations that underlie slow-channel myasthenic syndromes (SCCMS) or abbreviated channel activations that underlie the abnormally rapid decay of endplate currents in fast-channel syndromes (FCCMS). A third disorder associated with postsynaptic CMS is called CMS type Id (CMS1d), and could also result from mutations in the proteins forming the subunits of the muscle AChR. Mutations underlying SCCMS cause a gain of function and usually show dominant inheritance. & Defects in CHRND are a cause of fast-channel congenital myasthenic syndrome (FCCMS) [MIM:608930]. Mutations underlying FCCMS cause a loss of function and show recessive inheritance.
SIMILARITY: SwissProt: Q07001 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Protein Target:
hnAChR α1/β1/δ/ε
UniProt Number:
Target Sub-Family:
Other Ligand Gated
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- CHRNA1
- FCCMS
- ACHRA
- ACHRD
- CHRNA
- CHNRA
- CMS2A
- SCCMS
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hnAChR α1/β1/δ/ε-HEK Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- CHRNA1
- FCCMS
- ACHRA
- ACHRD
- CHRNA
- CHNRA
- CMS2A
- SCCMS