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PrecisION hCav2.2 (α1B/β3/α2δ1)-HEK Recombinant Cell Line
Description:
PrecisION hCav2.2 (α1B/β3/α2δ1)-HEK Recombinant Cell Line
Trade Name:
PrecisION
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the human Cav2.2 calcium ion channel
Background Information:
hCav2.2-α1B is a voltage-gated calcium channel alpha subunit. It is expressed almost exclusively in neurons typically located at high densities in presynaptic terminals. Opening of these channels enables the rapid influx of the extracellular Ca2+, triggering exocytotic vesicle fusion with terminal membranes and subsequent neurotransmitter release. Evidence suggests that Cav2.2 channels have a fundamental role in instigating increased excitability and neurotransmitter release in various pain syndromes. The link between Cav2.2 and pain neurotransmission in humans has been further vindicated by the effectiveness of PrialtR (ziconotide), a synthetic version of ω -conotoxin MVIIA, in treating severe chronic pain.
Application Notes:
hCav2.2 channels have been selectively expressed in a HEK293 cell line containing the pore forming α1B subunit and the auxiliary subunits β3 and α2δ1. They have been characterized in terms of their biophysical and pharmacological properties using IonWorksTM HT electrophysiology.
hCav2.2 channels activated at voltages positive to –15 mV and peak currents were obtained at around +10 mV to +20 mV. The V½ activation and V½ inactivation was around +13 mV (slope 7 mV) and –51 mV (slope 8.5) respectively. Currents were sensitive to the selective Cav2.2 channel blocker toxin ω-conotoxin GVIA (IC50 of 18 nM) but insensitive to the selective Cav1.2 blocker nitrendipine (IC50 >100 μM). Currents were also sensitive to the inorganic calcium channel blocker cadmium (IC50 of 7 μM).
hCav2.2 channels activated at voltages positive to –15 mV and peak currents were obtained at around +10 mV to +20 mV. The V½ activation and V½ inactivation was around +13 mV (slope 7 mV) and –51 mV (slope 8.5) respectively. Currents were sensitive to the selective Cav2.2 channel blocker toxin ω-conotoxin GVIA (IC50 of 18 nM) but insensitive to the selective Cav1.2 blocker nitrendipine (IC50 >100 μM). Currents were also sensitive to the inorganic calcium channel blocker cadmium (IC50 of 7 μM).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Protein Target:
hCav2.2 (α1B/β3/α2δ1)
Presentation:
2 x 1 ml aliquots
Target Sub-Family:
Calcium
Packaging:
2 x 1 ml aliquots
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hCav2.2 (α1B/β3/α2δ1)-HEK Recombinant Cell Line