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PrecisION hnAChR α3/β4-HEK Recombinant Cell Line
Description:
PrecisION hnAChR α3/β4-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the subunits α3 and β4 of the human nicotinic acetylcholine receptor.
Application Notes:
HEK293 cells expressing hnAChR α3/β4 were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques and FLIPR calcium assay. Using whole-cell patch clamp techniques, the cells responded to increasing concentrations of acetylcholine (ACh) in a dose-dependent manner up to 1 mM, after which desensitization occurred. The mean EC50 value for the ACh-elicited current was 140 ± 6.2 µM. 100 µM methyllycaconitine (MLA) completely blocked the current elicited by 300 µM ACh. In FLIPR calcium assays the agonists epibatidine, nicotine and cytisine had EC50 values of 81.4, 9.16 and 4.16 respectively. The antagonists mecamylamine, Dihydro-b-erythroidine (DHbE) and atropine blocked the EC80 acetylcholine agonist response with IC50 values of 481, 210 and 40 respectively. Functional channel expression over time was monitored using FLIPR calcium assays. Channel expression is robust over at least 35 passages. At passage 35 the fluorescence signal window in response to 83 µM epibatidine was >16,000 RFU with a Z’ value of approximately 0.9.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Protein Target:
hnAChR α3/β4
Target Sub-Family:
Other Ligand Gated
Gene Symbol:
CHRNA3
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hnAChR α3/β4-HEK Recombinant Cell Line