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PrecisION hTRPV1-HEK Recombinant Cell Line
Description:
PrecisION hTRPV1-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing human TRPV1 [capsaicin receptor / vanilloid receptor (VR1), accession number AF196175].
Format:
2 x 1 ml aliquots containing 3.80 x 106 cells/ml in 10% DMSO at passage 13
Format:
2 x 1 ml aliquots containing 3.80 x 106 cells/ml in 10% DMSO at passage 13
Background Information:
The TRPV1 (Transient Receptor Potential Vanilloid subtype 1) channels cation channels mediate neuronal responses to many noxious stimuli, including heat, low pH, neuropeptides and chemicals e.g. the capsaisin molecule responsible for the sensation of heat from chillies. This receptor may represent a novel target in the treatment of inflammatory pain.
Application Notes:
hTRPV1 channels have been selectively expressed in a HEK293 cell line and characterized in terms of their biophysical and pharmacological properties using whole-cell patch clamp techniques. hTRPV1 currents were activated by capsaicin in the same concentration range as reported previously (0.1-1 µM) and displayed outward rectification with a reversal potential close to 0 mV indicative of a non-selective cation current. hTRPV1 currents evoked by capsaicin were blocked by the competitive antagonist capsazepine (50 µM) and the more selective and potent competitive TRPV1 antagonist SB-366791 in the low nanomolar range. hTRPV1 current amplitudes, monitored using IonWorksTM HT, were consistently in excess of 2 nA and percent expression levels above 60% (currents greater than 500 pA) for greater than 40 cell passages. These data demonstrate the selective and stable functional expression of TRPV1 channels in this HEK293 cell line.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
UniProt Summary:
FUNCTION: SwissProt: Q8NER1 # Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
SIZE: 839 amino acids; 94938 Da
SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.
SIZE: 839 amino acids; 94938 Da
SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Protein Target:
hTRPV1
UniProt Number:
Target Sub-Family:
TRP
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- TRPV1
- VR1
- OTRPC1
- TrpV1
- DKFZp434K0220
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hTRPV1-HEK Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- TRPV1
- VR1
- OTRPC1
- TrpV1
- DKFZp434K0220