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PrecisION hTRPV3- HEK Recombinant Cell Line
Description:
PrecisION hTRPV3- HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing human TRPV3
Format:
2 x 1 ml aliquots containing 1.41 x 106cells/ml in 7.5% DMSO at passage 13.
Format:
2 x 1 ml aliquots containing 1.41 x 106cells/ml in 7.5% DMSO at passage 13.
Background Information:
The TRPV3 channel belongs to the transient receptor potential channel (TRP) super-family that consists of 7 subfamilies. The channels exist as tetramers where each subunit consists of 6 putative trans-membrane domains. In this respect they have a similar topology to many other voltage-gated channels. The vast majority are permeable to both monovalent cations and calcium (Clapham et al., 2001).
The hTRPV3 channel was first cloned in 2002 by two groups (Xu et al., 2002, Smith et al., 2002) based on its homology to known TRPV channels. It shares 40-50% homology to TRPV1 (Levine and Alessandri-Haber, 2007) and is activated at temperatures ≥ 34°C as well as by 2-Aminoethoxydiphenyl borate (2-APB, Hu et al., 2004) and monoterpenes such as camphor (Vogt-Eisele et al., 2007). In humans it is expressed in skin keratinocytes, trigeminal ganglia, spinal cord and brain (Xu et al., 2002). It is also located in DRG neurons (Xu et al., 2002, Smith et al., 2002, Facer et al., 2007) where it may even form heteromultimers with TRPV1 (Smith et al., 2002). The specific distribution of this channel, coupled with its thermal sensitivity and potential activation by a variety of inflammatory mediators, have suggested that it may represent a novel drug target for the treatment of inflammatory pain.
The hTRPV3 channel was first cloned in 2002 by two groups (Xu et al., 2002, Smith et al., 2002) based on its homology to known TRPV channels. It shares 40-50% homology to TRPV1 (Levine and Alessandri-Haber, 2007) and is activated at temperatures ≥ 34°C as well as by 2-Aminoethoxydiphenyl borate (2-APB, Hu et al., 2004) and monoterpenes such as camphor (Vogt-Eisele et al., 2007). In humans it is expressed in skin keratinocytes, trigeminal ganglia, spinal cord and brain (Xu et al., 2002). It is also located in DRG neurons (Xu et al., 2002, Smith et al., 2002, Facer et al., 2007) where it may even form heteromultimers with TRPV1 (Smith et al., 2002). The specific distribution of this channel, coupled with its thermal sensitivity and potential activation by a variety of inflammatory mediators, have suggested that it may represent a novel drug target for the treatment of inflammatory pain.
Application Notes:
hTRPV3 channels have been selectively expressed in a HEK293 cell line and characterized in terms of their biophysical and pharmacological properties using whole-cell patch clamp techniques.
hTRPV3 currents were activated by 2-Aminoethoxydiphenyl borate (2-APB, 300 μM) and successive applications of this concentration lead to progressive increases in current amplitude. The cells were also sensitive to camphor (2 mM) but only after prior sensitization by 2-APB. These effects are a distinguishing feature of TRPV3 channels. The I/V relationship, obtained with voltage ramps, was outwardly rectifying and reversed close to 0 mV. The cells were insensitive to high concentrations of capsaicin (10 μM) ruling out any contamination by TRPV1 currents.
hTRPV3 currents were blocked by ruthenium red at negative potentials but at positive potentials current potentiation was observed. These effects are consistent with previously published data. hTRPV3 current amplitudes, monitored using IonWorksTM HT, were consistently around 4 nA and percent expression levels above 90% (currents >500 pA) for at least 50 cell passages.These data demonstrate the selective and stable functional expression of TRPV3 channels in a HEK293 cell line.
hTRPV3 currents were activated by 2-Aminoethoxydiphenyl borate (2-APB, 300 μM) and successive applications of this concentration lead to progressive increases in current amplitude. The cells were also sensitive to camphor (2 mM) but only after prior sensitization by 2-APB. These effects are a distinguishing feature of TRPV3 channels. The I/V relationship, obtained with voltage ramps, was outwardly rectifying and reversed close to 0 mV. The cells were insensitive to high concentrations of capsaicin (10 μM) ruling out any contamination by TRPV1 currents.
hTRPV3 currents were blocked by ruthenium red at negative potentials but at positive potentials current potentiation was observed. These effects are consistent with previously published data. hTRPV3 current amplitudes, monitored using IonWorksTM HT, were consistently around 4 nA and percent expression levels above 90% (currents >500 pA) for at least 50 cell passages.These data demonstrate the selective and stable functional expression of TRPV3 channels in a HEK293 cell line.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels.
UniProt Summary:
FUNCTION: SwissProt: Q8NET8 # Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
SIZE: 790 amino acids; 90636 Da
SUBUNIT: May form a heteromeric channel with TRPV1. Interacts with TRPV1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
SIMILARITY: SwissProt: Q8NET8 ## Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
SIZE: 790 amino acids; 90636 Da
SUBUNIT: May form a heteromeric channel with TRPV1. Interacts with TRPV1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
SIMILARITY: SwissProt: Q8NET8 ## Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Protein Target:
hTRPV3
UniProt Number:
Target Sub-Family:
TRP
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- TRPV3
- TrpV3
- VRL3
- VRL-3
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hTRPV3- HEK Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- TRPV3
- TrpV3
- VRL3
- VRL-3