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PrecisION hKv2.1/hKv9.2- CHO Recombinant Cell Line
Description:
PrecisION hKv2.1/hKv9.2- CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing the human Kv7.3/Kv7.5 potassium ion channel
Application Notes:
CHO-K1 cells expressing hKv2.1/hKv9.2 were characterised in terms of their biophysical and pharmacological properties using whole-cell patch clamp techniques. The mean current amplitude at +60 mV was 4.9 ± 0.8 nA (n = 9) for hKv2.1/hKv9.2 (8.4 ± 3.1 nA (n = 6) for hKv2.1 when expressed alone). The threshold for current activation was between -20 and -30 mV. This property is characteristic of both hKv2.1 and hKv2.1/hKv9.2 currents described in the literature. The cell-line is shown to be expressing hKv2.1 current since it was inhibited by low mM concentrations of the potassium channel blocker tetraethylammonium (5 mM; 56.7 ± 1.2% inhibition, n = 7) which is similar to hKv2.1 when expressed alone (5 mM; 63.3 ± 1.1%, n = 4). The sensitivity of hKv2.1/hKv9.2 to low mM concentrations of the potassium channel blocker 4-aminopyridine was reduced (5 mM; 15.4 ± 2.3%, n=6) compared to that of hKv2.1 when expressed alone (1 mM; 33.8 ±1.5%, n = 3). This is in agreement with published findings that have shown that the sensitivity of Kv2.1 to 4-AP is greatly reduced (10-fold) when co-expressed with a Kv9.x subunits. Functional channel expression over time was monitored using IonWorksTM HT. Channel expression is robust over at least 35 passages: 95% of cells expressed outward current greater than 500 pA at passage 35 (n=98). The mean current amplitude of these cells was 1.44 nA. 64% of cells had a seal resistance of greater than 50 MOhm.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Protein Target:
hKv2.1/hKv9.2
Presentation:
2 x 1 ml aliquots
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv2.1/hKv9.2- CHO Recombinant Cell Line