Procedure for Coating Multiwell Dishes 1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
| Wells | _ | Amount/Well |
| 96 | | 100 μL |
| 48 | | 300 μL |
| 24 | | 0.5 mL |
| 12 | | 1.0 mL |
| 6 | | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.