Canine Adiponectin ELISA

Canine Adiponectin ELISA

Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism. Its expression is inversely correlated to insulin resistance. Adiponectin is also known by the following names: Acrp30, AdipoQ, APM-1 and GBP28. It circulates in µg/mL quantities and has a structural homology with type VIII collagen, hibernation protein, and C1q. In contrast to the majority of secreted proteins from adipose tissue which are elevated in obesity, adiponectin appears to be either decreased or unaltered with degree of adiposity. More intriguingly, adiponectin seems to ameliorate the obesity related risk factors unlike other adipose tissue secretory proteins which contribute toward the health risks associated with obesity, diabetes, and cardiovascular risks. Adiponectin also has an insulin sensitizing effect, making it an excellent candidate in drug development for obesity and diabetes. Circulating adiponectin levels seem to be an excellent biochemical marker for improved insulin resistance in obese and diabetic states.

ELISA

Room Temperature, 4 hour assay using 50 µL sample size (serum/plasma: 1:2000 dilution)

Canine

• Inter-assay: 0.5 - 9.0%
• Intra-assay: 2.9 – 4.2%

0.048 ng/mL

• Diabetes
• Obesity
• Metabolic Disorders

0.137 – 100 ng/mL adiponection

95.4–100.5%

Human Adiponectin - 33%
Rat Adiponectin - 100%
Mouse Adiponectin - Not Detectable
Cytokines (Mouse, Rat, Human) - Not Detectable

Endocrine Kits

• ADIPOQ
• ACDC
• ACRP30
• APM1
• GBP28

Colorimetric

88.2 - 100%

ELISA Assays