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  • : EZGRT-89K
  • : 96-well strip plate

Human Ghrelin (total) ELISA

Description:
Human Ghrelin (total) ELISA
Qty/Pk:
96-well strip plate
Background Information:
Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach.
Applications:
This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
Key Applications:
ELISA
Application Notes:
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Sales Restrictions:
This product is not available for sale in Japan.
Linearity of Dilution:
Serum: 95.7–104.7% Plasma: 95–117%
Sensitivity:
30 pg/mL (20 µL sample size)
Gene Symbol:
  • GHRL
  • ghrelin
  • obestatin
  • MTLRP
Product Name:
Human Ghrelin (total) ELISA
Specificity:
Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
Analytes Available:
Ghrelin (total)
UniProt Number:
Precision, %:
  • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
  • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
Accuracy:
  • Serum: (Active Ghrelin) 88–91%
  • (Des-octanoyl Ghrelin) 101–107%
  • Plasma: (Active Ghrelin) 84–87%
  • (Des-octanoyl Ghrelin) 112–119%
Detection Methods:
  • Fluorescent
  • Colorimetric
Entrez Gene Number: