Human Apo AIV ELISA

Human Apo AIV ELISA

Apolipoprotein AIV (Apo AIV) is a glycoprotein synthesized by the human intestine. Synthesis appears to be stimulated by the absorption of high density lipoproteins that are rich in triglycerides rather than by the uptake or re-esterification of fatty acids. The formation of chylomicrons acts as a signal for the induction of Apo AIV synthesis a signal enhanced by a factor from the ileum, probably PYY. Apo AIV alters the activity of the key enzymes (LPL and LCAT) of lipoprotein metabolism and cholesterol efflux from extrahepatic tissues. Also, since stimulation of intestinal synthesis and secretion by lipid absorption are rapid, Apo AIV likely plays a role in the short-term regulation of food intake. Other evidence suggests involvement in the long-term regulation of both food intake and body weight. Chronic ingestion of high-fat diet blunts the intestinal Apo AIV response to lipid feeding and, consequently, may explain why the chronic ingestion of a high-fat diet predisposes both animals and humans to obesity.

• Radioimmunoassay
• ELISA

This is a 4-hour assay at room temperature requiring 20 μL human serum, plasma, and tissue culture samples.

Human

• Cardiovascular Kits
• ELISA Assays

• Obesity
• Metabolic Disorders

77-100%

0.08 μg/mL

• Inter-assay 11 – 13%
• Intra-assay 4 – 6%

91-103%

Colorimetric

0.8 - 5.0 μg/mL

The antibodies used in this kit show a high cross-reactivity to non-human primate samples. However, no species cross-reactivity is obseved in the assay to rat, mouse, canine, porcine, or sheep samples.

No cross reactivity was observed to the following human analytes:
Apo A1, Apo AII, Apo AV, Apo B, Apo CII, Apo CIII, Apo E, cytokines, adipokines or endocrine hormones.

Apo AIV