Content Loading...
Content Loading...
Ordering Information
- : EZHAP0A4-73K
- : 96-well plate
Human Apo AIV ELISA
Description:
Human Apo AIV ELISA
Qty/Pk:
96-well plate
Background Information:
Apolipoprotein AIV (Apo AIV) is a glycoprotein synthesized by the human intestine. Synthesis appears to be stimulated by the absorption of high density lipoproteins that are rich in triglycerides rather than by the uptake or re-esterification of fatty acids. The formation of chylomicrons acts as a signal for the induction of Apo AIV synthesis a signal enhanced by a factor from the ileum, probably PYY. Apo AIV alters the activity of the key enzymes (LPL and LCAT) of lipoprotein metabolism and cholesterol efflux from extrahepatic tissues. Also, since stimulation of intestinal synthesis and secretion by lipid absorption are rapid, Apo AIV likely plays a role in the short-term regulation of food intake. Other evidence suggests involvement in the long-term regulation of both food intake and body weight. Chronic ingestion of high-fat diet blunts the intestinal Apo AIV response to lipid feeding and, consequently, may explain why the chronic ingestion of a high-fat diet predisposes both animals and humans to obesity.
Key Applications:
- Radioimmunoassay
- ELISA
Application Notes:
This is a 4-hour assay at room temperature requiring 20 μL human serum, plasma, and tissue culture samples.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Linearity of Dilution:
77-100%
Sensitivity:
0.08 μg/mL
Precision, %:
- Inter-assay 11 – 13%
- Intra-assay 4 – 6%
Accuracy:
91-103%
Detection Methods:
- Colorimetric
- Radioactive
Product Name:
Human Apo AIV ELISA
Standard Curve Range:
0.8 - 5.0 μg/mL
Specificity:
The antibodies used in this kit show a high cross-reactivity to non-human primate samples. However, no species cross-reactivity is obseved in the assay to rat, mouse, canine, porcine, or sheep samples.
No cross reactivity was observed to the following human analytes:
Apo A1, Apo AII, Apo AV, Apo B, Apo CII, Apo CIII, Apo E, cytokines, adipokines or endocrine hormones.
No cross reactivity was observed to the following human analytes:
Apo A1, Apo AII, Apo AV, Apo B, Apo CII, Apo CIII, Apo E, cytokines, adipokines or endocrine hormones.
Analytes Available:
Apo AIV