Human Apo AV ELISA
Description:
Human Apo AV ELISA
Background Information:
Apolipoprotein AV (Apo AV) is a newer member of the plasma apolipoprotein family. Mature human Apo AV is a 39 kD protein with 343 residues and displays 27% sequence identity with human A-IV. This protein is expressed in the liver and secreted into circulation with relatively low circulating levels compared to other apolipoproteins. Recent studies have demonstrated that Apo AV plays very important roles in the regulation of plasma triglyceride levels in rodents and humans.
Key Applications:
Radioimmunoassay
Application Notes:
20 µL sample size (serum /plasma 1:5 diluted)~3.5 hour assay, Room Temperature
Species Reactivity:
Human
Precision, %:
- Intra-assay 2.8 – 6.4%
- Inter-assay 5.3 – 12.4%
Sensitivity:
1.0 ng/mL
Therapeutic Areas:
- Cardiovascular Disease
- Metabolic Disorders
Standard Curve Range:
4.96 – 300 ng/mL
Accuracy:
78-105%
Specificity:
No species cross-reactivity is observed in the assay to rat, mouse, canine, porcine, or sheep Apo AV. However, there is a high degree of sample cross-reactivity observed to non-human primate samples which has not been explored or validated.
Kit Type:
Cardiovascular Kits
Gene Symbol:
- APOA5
- ApoA-V
- RAP3
- APOA-V
- MGC126838
- MGC126836
- ApoAV
- APOAV
Detection Methods:
Colorimetric
Linearity of Dilution:
85-117%
Assay Type:
Radioimmunoassays (RIA)