Human Insulin ELISA
Description:
Human Insulin ELISA
Background Information:
Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
Key Applications:
ELISA
Application Notes:
2 hour assay, room temperature Sample ≤ 20 µL serum, plasma, or tissue culture medium
Species Reactivity:
Human
Precision, %:
- Inter-assay: 9.1–11.4
- Intra-assay: 4.6–7.0
Descriptive Text:
The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Standard Curve Range:
2–200 µU/mL
Accuracy:
76–103%
Kit Type:
Endocrine Kits
Gene Symbol:
- INS
- proinsulin
- insulin
Analytes Available:
Insulin
Detection Methods:
Colorimetric
Linearity of Dilution:
96–112%
Kit or Assay Type:
- Endocrine Kits
- ELISA Assays
Assay Type:
ELISA Assays