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Ordering Information
- : EZHIASF-14K
- : 96-well strip plate
Human Insulin ELISA, animal serum free
Description:
Human Insulin ELISA, animal serum free
Qty/Pk:
96-well strip plate
Background Information:
This unique Human Insulin Elisa kit is animal serum free–the monoclonal antibodies are harvested from clones grown in culture medium devoid of any animal protein.
Key Applications:
ELISA
Application Notes:
3.5 hour assay, room temperature, 20 µL sample volume
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Linearity of Dilution:
73–100%
Sensitivity:
0.73 µU/mL
UniProt Number:
Precision, %:
- Inter-assay: Coefficient of Variation:6.0–7.1
- Intra-assay: Coefficient of Variation: 1.7–1.9
Accuracy:
88–128%
Detection Methods:
- Fluorescent
- Colorimetric
Gene Symbol:
- INS
- proinsulin
- insulin
Product Name:
Human Insulin ELISA, animal serum free
Standard Curve Range:
2–200 µU/mL
Entrez Gene Number:
Specificity:
No Cross-reactivity with Human Pro-insulin or Human C-peptide
Analytes Available:
Insulin