Human Leptin "Dual Range" ELISA
Description:
Human Leptin "Dual Range" ELISA
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
ELISA
Application Notes:
Room temperature, 3.5 hour assay 25 µL sample size (Sensitive Assay: 50µL)
Species Reactivity:
Human
Precision, %:
- Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
- Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
Sensitivity:
- 0.5 ng/mL
- Sensitive Assay 0.125 ng/mL
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Standard Curve Range:
- 0.5–100 ng/mL
- Sensitive Assay 0.125–20 ng/mL
Accuracy:
- 94–114%
- Sensitive Assay 86–102%
Kit Type:
Endocrine Kits
Gene Symbol:
- LEP
- leptin
- OB
- obesity
- OBS
Detection Methods:
Colorimetric
Linearity of Dilution:
- 80–105%
- Sensitive Assay 96–125%
Kit or Assay Type:
- Endocrine Kits
- ELISA Assays
Assay Type:
ELISA Assays