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Human Leptin "Dual Range" ELISA


Description:
Human Leptin "Dual Range" ELISA
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
ELISA
Application Notes:
Room temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)
Species Reactivity:
Human
Kit or Assay Type:
  • Endocrine Kits
  • ELISA Assays
Therapeutic Areas:
  • Obesity
  • Metabolic Disorders
Linearity of Dilution:
  • 80–105%
  • Sensitive Assay 96–125%
Sensitivity:
  • 0.5 ng/mL
  • Sensitive Assay 0.125 ng/mL
Precision, %:
  • Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
  • Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
Accuracy:
  • 94–114%
  • Sensitive Assay 86–102%
Detection Methods:
Colorimetric
Gene Symbol:
  • LEP
  • leptin
  • OB
  • obesity
  • OBS
Standard Curve Range:
  • 0.5–100 ng/mL
  • Sensitive Assay 0.125–20 ng/mL
Analytes Available:
Leptin

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