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Analyzed Dual Parameter Data: Dot plots depicting Jurkat cells treated with multiple inducers and stained using MitoDamage Kit. Jurkat cells were tr...
FlowCellect™ MitoDamage Kit
Description:
FlowCellect MitoDamage Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests
Product Overview:
Millipore’s FlowCellect™MitoDamage Kit includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria, and is responsive to mitochondrial potential changes. Annexin V conjugated to a green sensitive dye, CF488A, which binds to phosphatidylserine (PS) on the surface of apoptotic cells. And the cell impermeant DNA intercalator 7-Aminoactinomycin-D (7-AAD), a dead cell dye. The simultaneous use of the reagents allows researchers to obtain information on early, mid and late apoptosis in one simple assay.
MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF488A which is excited by a 488 nm laser and emits at 525 nm. Control cells depict no green fluorescence while apoptotic cells exhibit positive green fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells and these cells have low red fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with intact mitochondrial membrane 2) Cells with dissipated membrane potential but no Annexin V or 7AAD staining 3) Early apoptotic cells with dissipated membrane potential and Annexin V binding 4) Late Apoptotic Cells or dead cells with dissipated membrane potentials. The kit thereby provides a complete picture of mitochondrial health and cell health and allows the correlation of mitochondrial damage to cell health. The entire assay can be performed in less than30 minutes once cellular samples are ready.
MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF488A which is excited by a 488 nm laser and emits at 525 nm. Control cells depict no green fluorescence while apoptotic cells exhibit positive green fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells and these cells have low red fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with intact mitochondrial membrane 2) Cells with dissipated membrane potential but no Annexin V or 7AAD staining 3) Early apoptotic cells with dissipated membrane potential and Annexin V binding 4) Late Apoptotic Cells or dead cells with dissipated membrane potentials. The kit thereby provides a complete picture of mitochondrial health and cell health and allows the correlation of mitochondrial damage to cell health. The entire assay can be performed in less than30 minutes once cellular samples are ready.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- MitoSense Red Dye
- Annexin CF 488
- 7-AAD
- 10X Assay Buffer
- User Manual
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ MitoDamage Kit
Alternate Names:
FlowCellect
Flow Cytometry Software Type:
inCyte
Flow Cytometry Market:
- Bioprocess
- Research
Flow Cytometry Research Subcategory:
Early