FlowCellect Chemokine Receptor CCR1 Surface Expression Identification and Quantification Kit

FlowCellect Chemokine Receptor CCR1 Surface Expression Identification and Quantification Kit

FlowCellect

100 Tests / Kit

Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Also included in the kit is a secondary antibody conjugate to R-Phycoerythrin, which is a bright fluorochrome ideal for measuring endogenous levels of GPCR expression on native cells. By following the steps outlined in the assay procedure, researchers can easily identify which native cell lines would be suitable for performing secondary assays in support of their structure-activity drug discovery efforts.

Additional test applications include the identification of high, medium, and low expressing cell cultures during the clonal selection process. When a researcher decides to build a stable cell line in-house, it is essential that the clone of choice will provide the GPCR surface expression needed to perform drug screening. Choosing the wrong clone can adversely affect a primary screen if the chosen clone cannot detect lower affinity compounds. This can lead to a lower hit rate, which can result in screening limitations when identifying potential blockbuster compounds.

The FlowCellect™ GPCR identification kits are designed for flow cytometry applications, which can be used for the identification and quantification of the surface expression level of GPCRs on any cells. The kit uses a GPCR antibody validated for flow cytometry to detect receptor expression, and also includes both positive and negative control cells with well characterized receptor expression levels for the purpose of quantification.

Chemokine receptors are very attractive targets because they are highly expressed on immune cells and regulate leukocyte activation and trafficking. Many of these targets have been indicated to play a key role in certain diseases states such as HIV infection, Rheumatoid Arthritis, and Diabetes. The expression levels of these receptors often reflect the different states of infectious diseases and inflammation.

Chemokine receptors are seven transmembrane G-Protein coupled receptors (GPCRs). The chemokine receptor family comprises of eighteen GPCR targets, in which ten are CC chemokine receptors, seven are CXC chemokine receptors, and one is a CX3C chemokine receptor. Currently, Millipore has launched 12 GPCR surface identification kits with an initial focus on the chemokine receptor family.

CCR1 is a member of the chemokine receptor family; the ligands for CCR1 include MIP-1, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 [8]. CCR1 is widely expressed in many types of hematopoietic cells such as lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 [2, 6]. Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection [3, 4, 5], allergic encephalomyelitis [7], and renal fibrosis [1] in experimental models.

The control cells provided in the kit are based on the commercially available ChemiScreen™ GPCR stable cell lines from Millipore. Both the positive and negative control cell lines have been fully characterized using Pharmacology techniques such as radioligand binding assay to determine the receptor number for each cell type [9]. Using this information, now the researcher can determine the number of receptors per cell on any given cell type.

• Flow Cytometry
• in vitro Toxicology Assays

Human

• Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
• Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Lot to lot in-process testing on all kit components

• Signaling
• Chemokine Analysis

• 1. 500X Mouse anti-human CCR1 Monoclonal Antibody
• Clone ID 53504: - (Part No. CS202128) One 20uL vial
• 2. 500X Secondary Antibody conjugated to R-Phycoerythrin: - (Part No. CS202129) One 20L vial
• 3. Antibody Diluent: - (Part No. CS202130) One 1mL vial
• 4. Fixation Buffer: - (Part No. CS202122) One 13mL bottle
• 5. 10X Wash Buffer: - (Part No. CS202123) One 13mL bottle
• 6. 5X Assay Buffer: - (Part No. CS202124) One 55mL bottle
• 7. Positive Control Cell Culture for CCR1 : - (Part No. CS202131) One vial supplied with 4x106 cells
• 8. Negative Control Cell Culture for CCR1 : - (Part No. CS202121) One vial supplied with 4x106 cells

4-8°C for antibodies and buffers; -80°C for positive and negative control cells; 6 Months

Mouse

Get 30% off on Your Purchase of Any of Our Flow Cytometry Kits & Reagents Today!
Reference Promo Code ZBIOS406 when placing your order*.

*This offer is valid until September 30, 2011. Guava Kits with catalogue numbers that begin with the number 4 are excluded from this promotion. Discount can not be combined with any other discount.

• Fluorescent
• ¹²⁵I

FlowCellect Chemokine Receptor CCR1 Surface Expression Identification and Quantification Kit

Flow Cellect

ExpressPro

Monoclonal Antibody

Research