Product Images
Flow Cytometry Analysis:
Representative lot data.
FACS analysis of human peripheral blood lymphocytes stained with α-Human CD3, clone UCHT...
Milli-Mark Anti-CD3 -PECy5, clone UCHT1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | Phycoerythrin/Cy5 | Monoclonal Antibody |
Description:
Milli-Mark Anti-CD3 -PECy5 Antibody, clone UCHT1 | FCMAB169C5
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Human CD3 antigen.
Molecular Weight:
20 kDa (Campana, 1987)
Immunogen:
Human infant thymocytes and lymphocytes from a patient with Sézary disease.
Clone:
UCHT1
Isotype:
IgG1κ
Background Information:
Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).
Species Reactivity:
Human
Species Reactivity Note:
Human.
Control:
Human Lymphocytes
Quality Assurance:
Evaluated by flow cytometry using Human Lymphocytes.
Purification Method:
Protein A purfied
Presentation:
Purified Mouse monoclonal IgG1 conjugated to PE/Cy5 in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Alternate Names:
T-cell receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Product Name:
Milli-Mark Anti-CD3 -PECy5, clone UCHT1
Concentration:
0.1 mg/mL mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Phycoerythrin/Cy5
Host:
Mouse