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  • : FTLV00003
  • : 3 purifications and concentrations
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Fast-Trap Lentivirus Purification and Concentration Kit

Description:
Fast-Trap Lentivirus Purification and Concentration Kit
Trade Name:
Fast-Trap
Qty/Pk:
3 purifications and concentrations
Product Overview:
The Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of lentivirus, up to capacity of approximately 2x108 total infectious lentivirus particles.

The purification kit protocol calls for collecting the supernatant from transfected cells that have released the virus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit.

For laboratory use only; not for diagnostic or in vivo use.
Key Applications:
Lentivirus Purification and Concentration
Usage Statement:
General Lab Use
Components:
3 – Fast-Trap virus filter units
3 – Steriflip-HV filter units
1 – 10x Binding buffer, 20 mL
1 – Equilibration buffer, 100 mL
1 – Wash buffer, 200 mL
1 – Elution buffer, 21 mL
3 – Amicon Ultra-4 filter units, 100 kDa NMWL
1 – Test tube stand
1 – User Guide
1 – Overview of Procedure card
1 – Material Safety Data Sheet
Storage Conditions:
The Fast-Trap Lentivirus Purification and Concentration Kit should be stored at room temperature.

Warnings and Precautions:

WARNING: This kit permits quick purification of lentivirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit.

WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet.
For research use only. Not for use in diagnostic or human clinical procedures.
Variation in the amount of lentivirus purified with this kit may be due in part to infection success and cell conditions.
Packaging:
3 purifications and concentrations
Product Name:
Fast-Trap Lentivirus Purification and Concentration Kit

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