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  • : GHRT-89HK
  • : 125 tubes

Human Ghrelin (TOTAL) RIA

Description:
Human Ghrelin (TOTAL) RIA
Qty/Pk:
125 tubes
Background Information:
Ghrelin is a recently discovered hormone is secreted primarily by the stomach in response to glucose administration or CNS signals.
Applications:
This radioimmunoassay (RIA) measures & quantifies Ghrelin for use in Metabolism & Endocrine & Neuroscience research.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium assay at 4°C. Sample volume: < 100 μL Plasma (collected on ice) recommended
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Linearity of Dilution:
99–146%
Label:
125I-Human Ghrelin
Sensitivity:
100–10,000 pg/mL
Gene Symbol:
  • GHRL
  • ghrelin
  • MTLRP
  • obestatin
Product Name:
Human Ghrelin (TOTAL) RIA
Analytes Available:
Ghrelin
UniProt Number:
Precision, %:
  • Inter-assay: 14.7–17.8
  • Intra-assay: 3.3–10.0
Accuracy:
90–96%
Detection Methods:
  • Radioactive
  • 125I
Performance:
  • ED80 = 395 ± 88 pg/mL
  • ED50 = 895 ± 177 pg/mL
  • ED20 = 2015 ± 382 pg/mL
Entrez Gene Number:

Product Resources

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