Glucagon RIA
Description:
Glucagon RIA
Background Information:
Glucagon is a 3,5 kDa hormone secreted by the A cells of the Islets of Langerhans in the pancreas and is involved in regulation of glucose homeostasis thro µgh enhanced synthesis and mobilization of glucose in the liver, and regulation of glucose-stimulated insulin secretion.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume < 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Mammals
Precision, %:
- Inter-assay: 11.7 ± 3.0
- Intra-assay: 4.9 ± 1.3
Descriptive Text:
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Accuracy:
97 ± 0.8%
Standard Curve Range:
20–400 pg/mL
Label:
125I-Glucagon
Kit Type:
Endocrine Kits
Gene Symbol:
- GCG
- GLP1
- glucagon
- GRPP
- GLP2
Analytes Available:
Glucagon
Detection Methods:
125I
Cross Reactivity:
Glucagon is conserved in almost all mammals. This kit may be used to measure glucagon in these samples (e.g. rat, mouse, etc.)
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Performance:
- ED80 = 44 ± 4 pg/mL
- ED50 = 109 ± 5 pg/mL
- ED20 = 264 ± 17 pg/mL
Assay Type:
Radioimmunoassays (RIA)