Ordering Information
Glucagon RIA
Description:
Glucagon RIA
Background Information:
Glucagon is a 3,5 kDa hormone secreted by the A cells of the Islets of Langerhans in the pancreas and is involved in regulation of glucose homeostasis thro µgh enhanced synthesis and mobilization of glucose in the liver, and regulation of glucose-stimulated insulin secretion.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume < 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Mammals
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
- Obesity
- Metabolic Disorders
Label:
125I-Glucagon
Precision, %:
- Inter-assay: 11.7 ± 3.0
- Intra-assay: 4.9 ± 1.3
Accuracy:
97 ± 0.8%
Detection Methods:
125I
Gene Symbol:
- GCG
- GLP1
- glucagon
- GRPP
- GLP2
Performance:
- ED80 = 44 ± 4 pg/mL
- ED50 = 109 ± 5 pg/mL
- ED20 = 264 ± 17 pg/mL
Cross Reactivity:
Glucagon is conserved in almost all mammals. This kit may be used to measure glucagon in these samples (e.g. rat, mouse, etc.)
Standard Curve Range:
20–400 pg/mL
Analytes Available:
Glucagon