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Human Adiponectin RIA
Description:
Human Adiponectin RIA
Background Information:
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight incubation at room temperature.
Sample volume <2 μL serum or plasma, or <100 μL tissue culture media
Sample volume <2 μL serum or plasma, or <100 μL tissue culture media
Species Reactivity:
Human
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
- Osteoporosis
- Arthritis
- Chronic Inflammatory Disease
- Bone Metastasis
Linearity of Dilution:
85–119%
Label:
125I-Adiponectin
Sensitivity:
0.78 ng/mL
Precision, %:
- Inter-assay: 6.9 – 9.3 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL)
- Intra-assay: 1.8 – 6.2 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL)
Accuracy:
- 116% (serum sample concentration 1:500 dilution–5 ng/mL standard)
- 111% (serum sample concentration 1:500 dilution–20 ng/mL standard)
- 101% (serum sample concentration 1:500 dilution–100 ng/mL standard)
Detection Methods:
125I
Gene Symbol:
- ADIPOQ
- AdipoQ
- apM-1
- APM1
- GBP28
- ACRP30
- apM1
- adiponectin
- ACDC
- APM-1
Performance:
ED80 = 3.9 ± 0.9 ng/mL
ED50 = 21.0 ± 5.7 ng/mL
ED20 = 128.0 ± 48.6 ng/mL
ED50 = 21.0 ± 5.7 ng/mL
ED20 = 128.0 ± 48.6 ng/mL
Standard Curve Range:
0.78–200 ng/mL
Specificity:
Human Adiponectin 100%
C1q Human < 0.01%~
Mouse Adiponectin 400%
C1q Human < 0.01%~
Mouse Adiponectin 400%
Analytes Available:
Adiponectin