MILLIPLEX MAP Human RANKL - Single Plex - Metabolism Assay

MILLIPLEX MAP Human RANKL - Single Plex

MILLIPLEX

96-well plate

RANKL (receptor activator of nuclear factor-kappaB ligand; also know as RANK Ligand and RANCE) is a member of the TNFsuperfamily and plays a key role in the formation and actvation of osteoclasts. Two isoforms of RANKL are generated by alternate splicing, a membrane-bound RANKL and a secreted soluble form RANKL(sRANKL). RANKL is secreted by cells of oesteoblastic lineage, endothelial and activated T lymphocytes. This single plex assay kit is to be used for the quantitative measurement of soluble human RANKL in cell/tissue culture supernatant samples and diluted human serum or plasma samples

This MILLIPLEX® MAP Human RANKL - Single Plex is a Metabolism single plex assay. The analyte available for this kit is RANKL.

Multiplexing

25 µL or less sample volume. Overnight assay (1:2 dilution for serum & plasma samples)

Human

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This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

FUNCTION: SwissProt: O14788 # Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
SIZE: 317 amino acids; 35478 Da
SUBUNIT: Homotrimer (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type II membrane protein. & Isoform 3: Cell membrane; Single-pass type II membrane protein. & Isoform 2: Secreted (By similarity). & Processed isoform 1: Secreted (By similarity).
TISSUE SPECIFICITY: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
DISEASE: "SwissProt: O14788 # Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non- functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development."
SIMILARITY: SwissProt: O14788 ## Belongs to the tumor necrosis factor family.

121.7%

5.0 pg/mL

• TNFSF11
• RANKL
• hRANKL2
• ODF
• OPGL
• CD254
• OPGLG
• sOdf
• OPTB2
• TRANCE

Human RANKL assay shows no cross reactivity with mouse and rat RANKL

MILLIPLEX MAP Human RANKL - Single Plex

5 - 20,000 pg/mL

RANKL

Single Plex

O14788

Non-Magnetic Bone

• Intra-assay:< 5%
• Inter-assay:<6%

93.2%

• Fluorescent
• Luminex xMAP

• NM_003701.2
• NM_033012.2

Everything you need in a single kit.