Ordering Information
- : HGAMMAG-303E
- : 1 kit
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MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
Description:
MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit, HGAMMAG-303E
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Background Information:
Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found
primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.
Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.
Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
Applications:
This MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit is an Isotyping assay. The analyte available for this kit is IgE.
Key Applications:
Multiplexing
Application Notes:
• This kit may be used for the analysis of IgE in human serum, plasma or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sensitivity:
0.4 ng/mL
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Standard Curve Range:
IgE 4.1 – 1,000 ng/mL
Product Name:
MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit, HGAMMAG-303E
Specificity:
Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Analytes Available:
IgE
Configuration:
Single Plex
Components:
- MILLIPLEX® MAP Anti-Human k and λ Light Chain, PE
- MILLIPLEX® MAP Human IgE Standard
- MILLIPLEX® MAP Human Serum Positive Control
- MILLIPLEX® MAP Assay Buffer
- MILLIPLEX® MAP Washer Buffer, 10X
- Set of one 96-Well Filter Plate with 4 Sealers
Panel Type:
MAGNETIC Immune Response
Precision, %:
- Intra-Assay < 10
- Inter-Assay < 13
Accuracy:
IgE 102%
Detection Methods:
Luminex xMAP
Packaging:
96 Well Plate