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Ordering Information
- : HI-11K
- : 250 tubes
Ultra Sensitive Human Insulin RIA
Description:
Ultra Sensitive Human Insulin RIA
Qty/Pk:
250 tubes
Background Information:
Insulin, a 5.8 kDa hormone is secreted by the islet beta cells in the pancreas. This kit measures human insulin with high sensitivity but minimum cross-activity to proinsulin
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at room temperature. Sample volume 10 to 100 µL serum, plasma, or tissue culture media
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Label:
125I-Insulin
UniProt Number:
Detection Methods:
- Radioactive
- 125I
Gene Symbol:
- INS
- proinsulin
- insulin
Performance:
- ED80 = 0.9 ± 0.1 µU/ml
- ED50 = 3.1 ± 0.3 µU/ml
- ED20 = 12.1 ± 2.2 µU/ml
Product Name:
Ultra Sensitive Human Insulin RIA
Standard Curve Range:
0.2–20 µU/mL
Entrez Gene Number:
Specificity:
Human Insulin 100% Intact Human Proinsulin 6% Des 31, 32 HPI 6% Des 64, 65 HPI 78% Bovine Insulin 42% Bovine Proinsulin <2% Porcine Insulin 90% Porcine Proinsulin <5% Rat Insulin <1.0% IGF-1 Not Detectable Glucagon Not Detectable Somatostatin Not
Analytes Available:
Insulin