Ordering Information

Catalogue Number : HL-81HK
Qty/Pk : 125 tubes

Human Leptin RIA

Description:

Human Leptin RIA

Qty/Pk:

125 tubes

Background Information:

Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.

Key Applications:

Radioimmunoassay

Application Notes:

Overnight, equilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.

Species Reactivity:

Human

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.

UniProt Summary:

FUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.

Linearity of Dilution:

88–101%

Label:

¹²⁵I-Human Leptin

UniProt Number:

P41159

Precision, %:

Inter-assay: 3.6–6.2
Intra-assay: 3.4–8.3

Accuracy:

103–105%

Detection Methods:

Radioactive
¹²⁵I

Gene Symbol:

LEP
leptin
OB
obesity
OBS

Performance:

ED₈₀ = 1.2 ± 0.1 ng/ml
ED₅₀ = 5.1 ± 0.4 ng/ml
ED₂₀ = 23.7 ± 2.1 ng/ml

Product Name:

Human Leptin RIA

Standard Curve Range:

0.5–100 ng/mL

Entrez Gene Number:

NM_000230.1

Specificity:

Human Leptin 100% Rat leptin <0.2% Mouse Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I Not Detectable, Somatostatin, PP Not Detectabl Rat Insulin Not Detectable Human Proinsulin Not Detectabl Canine Lepti

Analytes Available:

Leptin