Human Proinsulin RIA

Human Proinsulin RIA

Proinsulin is made by the beta cells of the pancreas

Radioimmunoassay

Three-day, disequilibrium incubation at room temperature. Sample volume ≤200 µL serum, plasma, or tissue culture media.

Human

• Inter-assay: 1.5–10.1
• Intra-assay: 1.5–6.9

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

Inflammation

91–93%

2–100 pM

¹²⁵I-Human Proinsulin

Intact Human Proinsulin 100% Des 31, 32 HPI 95% Des 64, 65 HPI <0.1% Bovine, Porcine Proinsulin Not Detectable Human Insulin <0.1% Human C-Peptide <0.1% Glucagon Not Detectable Somatostatin, PP Not Detectable Human IGF-I Not Detectable Human IGF

Endocrine Kits

• INS
• proinsulin
• insulin

Proinsulin

¹²⁵I

100–120%

• Endocrine Kits
• Radioimmunoassays (RIA)

• ED₈₀ = 10 ± 1 pM
• ED₅₀ = 23 ± 2 pM
• ED₂₀ = 52 ± 3 pM

Radioimmunoassays (RIA)