Ordering Information
Human Proinsulin RIA
Description:
Human Proinsulin RIA
Background Information:
Proinsulin is made by the beta cells of the pancreas
Key Applications:
Radioimmunoassay
Application Notes:
Three-day, disequilibrium incubation at room temperature. Sample volume ≤200 µL serum, plasma, or tissue culture media.
Species Reactivity:
Human
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
Inflammation
Linearity of Dilution:
100–120%
Label:
125I-Human Proinsulin
Precision, %:
- Inter-assay: 1.5–10.1
- Intra-assay: 1.5–6.9
Accuracy:
91–93%
Detection Methods:
125I
Gene Symbol:
- INS
- proinsulin
- insulin
Performance:
- ED80 = 10 ± 1 pM
- ED50 = 23 ± 2 pM
- ED20 = 52 ± 3 pM
Standard Curve Range:
2–100 pM
Specificity:
Intact Human Proinsulin 100% Des 31, 32 HPI 95% Des 64, 65 HPI <0.1% Bovine, Porcine Proinsulin Not Detectable Human Insulin <0.1% Human C-Peptide <0.1% Glucagon Not Detectable Somatostatin, PP Not Detectable Human IGF-I Not Detectable Human IGF
Analytes Available:
Proinsulin