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Ordering Information
- : HPI-15K
- : 250 tubes
Human Proinsulin RIA
Description:
Human Proinsulin RIA
Qty/Pk:
250 tubes
Background Information:
Proinsulin is made by the beta cells of the pancreas
Key Applications:
Radioimmunoassay
Application Notes:
Three-day, disequilibrium incubation at room temperature. Sample volume ≤200 µL serum, plasma, or tissue culture media.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Linearity of Dilution:
100–120%
Label:
125I-Human Proinsulin
UniProt Number:
Precision, %:
- Inter-assay: 1.5–10.1
- Intra-assay: 1.5–6.9
Accuracy:
91–93%
Detection Methods:
- Radioactive
- 125I
Gene Symbol:
- INS
- proinsulin
- insulin
Performance:
- ED80 = 10 ± 1 pM
- ED50 = 23 ± 2 pM
- ED20 = 52 ± 3 pM
Product Name:
Human Proinsulin RIA
Standard Curve Range:
2–100 pM
Entrez Gene Number:
Specificity:
Intact Human Proinsulin 100% Des 31, 32 HPI 95% Des 64, 65 HPI <0.1% Bovine, Porcine Proinsulin Not Detectable Human Insulin <0.1% Human C-Peptide <0.1% Glucagon Not Detectable Somatostatin, PP Not Detectable Human IGF-I Not Detectable Human IGF
Analytes Available:
Proinsulin