LisPro Insulin RIA

LisPro Insulin RIA

Lispro insulin (Humalog®) is a synthetic human insulin analog showing drastic reductions of inter-molecular aggregations. It has a higher rate of absorption, higher peak serum levels and a shorter duration of action than regular human insulin following s

Radioimmunoassay

Overnight assay Equilibrium incubation at room tempature. Sample volume 100 µL serum, plasma, or tissue culture media

Human

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

• sVCAM-1 33.0
• sICAM-1 30.0
• sFas 7.0
• sFasL 6.0
• MIF 10.0
• PAI-1 3.2

• Diabetes
• Obesity
• Metabolic Disorders

2.5–250 µU/mL

¹²⁵I-LisPro Insulin

Lis/Pro Insulin 100% Human Insulin <0.05% Human Proinsulin <0.05% Bovine Proinsulin Not Detectable Porcine Proinsulin Not Detectable

Endocrine Kits

• INS
• proinsulin
• insulin

Insulin

¹²⁵I

• Endocrine Kits
• Radioimmunoassays (RIA)

• ED₈₀ = 10 ± 1 µU/ml
• ED₅₀ = 36 ± 2 µU/ml
• ED₂₀ = 136 ± 5 µU/ml

Radioimmunoassays (RIA)