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  • : LPI-16K
  • : 250 tubes

LisPro Insulin RIA

Description:
LisPro Insulin RIA
Qty/Pk:
250 tubes
Background Information:
Lispro insulin (Humalog®) is a synthetic human insulin analog showing drastic reductions of inter-molecular aggregations. It has a higher rate of absorption, higher peak serum levels and a shorter duration of action than regular human insulin following s
Applications:
This radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight assay Equilibrium incubation at room tempature. Sample volume 100 µL serum, plasma, or tissue culture media
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Label:
125I-LisPro Insulin
Sensitivity:
  • sVCAM-1 33.0
  • sICAM-1 30.0
  • sFas 7.0
  • sFasL 6.0
  • MIF 10.0
  • PAI-1 3.2
Gene Symbol:
  • INS
  • proinsulin
  • insulin
Product Name:
LisPro Insulin RIA
Standard Curve Range:
2.5–250 µU/mL
Specificity:
Lis/Pro Insulin 100% Human Insulin <0.05% Human Proinsulin <0.05% Bovine Proinsulin Not Detectable Porcine Proinsulin Not Detectable
Analytes Available:
Insulin
UniProt Number:
Detection Methods:
  • Radioactive
  • 125I
Performance:
  • ED80 = 10 ± 1 µU/ml
  • ED50 = 36 ± 2 µU/ml
  • ED20 = 136 ± 5 µU/ml
Entrez Gene Number: