Ordering Information
- : MAB1098
- : 200 µg
Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 | MAB1098
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IHC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 | MAB1098
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody is use for detection, identification and neutralixzation of TNF-b in cell culture and other biological samples. The antibody reacts with human natural and recombinant TNF-b (lymphotoxin). It does not react with human TNF-a. Determination of cross-reactivity to TNF-b from other species has not been done.
Immunogen:
Human, recombinant tumor necrosis factor-beta (TNF-beta, lymphotoxin) produced in E. Coli.
Clone:
9B9
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Western blot
Immunocytochemistry
Immunohistochemistry
Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry
Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .
Optimal working dilutions must be determined by end user.
Presentation:
Purified immunoglobulin (Protein A-agarose chormotography). One bottle of lyophilized preparation contains:200ug mouse IgG, 2 mg potassium phosphate buffer, 2.9 mg sodium chloride, and 20 mg raffinose.Reconstitution with 1 ml sterile, distilled water results in an antibody concentration of 200 ug/ml.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.Freeze only once.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- LTA
- OTTHUMP00000037612
- TNF-beta
- OTTHUMP00000037613
- LT-alpha
- LT
- TNFB
- TNFSF1
Alternate Names:
TNF-beta
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
UniProt Summary:
FUNCTION: SwissProt: P01374 # Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
SIZE: 205 amino acids; 22297 Da
SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits.
SUBCELLULAR LOCATION: Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated.
DISEASE: SwissProt: P01374 # Genetic variations in LTA are associated with susceptibility to leprosy type 4 (LPRS4) [MIM:610988]; also known as susceptibility to age-dependent leprosy. Leprosy is a chronic infectious disease of peripheral sensory nerves caused by Mycobacterium leprae. & Genetic variations in LTA are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.
SIMILARITY: SwissProt: P01374 ## Belongs to the tumor necrosis factor family.
SIZE: 205 amino acids; 22297 Da
SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits.
SUBCELLULAR LOCATION: Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated.
DISEASE: SwissProt: P01374 # Genetic variations in LTA are associated with susceptibility to leprosy type 4 (LPRS4) [MIM:610988]; also known as susceptibility to age-dependent leprosy. Leprosy is a chronic infectious disease of peripheral sensory nerves caused by Mycobacterium leprae. & Genetic variations in LTA are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.
SIMILARITY: SwissProt: P01374 ## Belongs to the tumor necrosis factor family.
Brand Family:
Chemicon
Product Name:
Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 | MAB1098
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Mouse