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Ordering Information

  • : MAB1281
  • : 100 μL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

Mouse anti-Human Nuclei (Catalogue No. MAB1281) staining of human neural stem cells transplanted into rat brain. Photo courtesy of ReNeuron.

Anti-Nuclei Antibody, clone 235-1

Species Reactivity Key Applications Host Format Antibody Type
Human Only IH(P), IP, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-Nuclei Antibody, clone 235-1
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
Clone:
235-1
Isotype:
Background Information:
Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
Species Reactivity:
Human Only
Species Reactivity Note:
The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.
Application Notes:
Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

Identifies an 82 and 70 kD protein by immunoprecipitation.

Optimal working dilutions and protocols must be determined by the end user.
Control:
All human cell types
Purification Method:
Protein A purfied
Presentation:
Hybridoma supernatant. Liquid in PBS. Contains no preservative.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Alternate Names:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Immunocytochemistry
Brand Family:
Chemicon
Product Name:
Anti-Nuclei Antibody, clone 235-1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
Purified
Host:
Mouse
Applications:
Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.

Product Resources

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