Product Images
Immunocytochemistry Analysis:
Confocal IF analysis of Hela, NIH/3T3 using anti-Actin Mouse mAB (Red). Actin filaments have been labeled with Ale...
Western Blot Analysis:
HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Actin, clone C4. Proteins wer...
Anti-Actin, Clone C4
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, ICC, IHC, IH(P), WB | Mouse | Protein G Purified | Monoclonal Antibody |
Description:
Anti-Actin Antibody, near a.a. 50-70, clone C4 | MAB1501R
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
MAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Molecular Weight:
43 kDa
Epitope:
a.a. 50-70
Immunogen:
Purified chicken gizzard actin (Lessard, 1988).
Clone:
C4
Isotype:
IgG1κ
Background Information:
Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
Species Reactivity:
All
Species Reactivity Note:
To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
Application Notes:
Immunocytochemistry:
10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).
Immunohistochemistry:
10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.
ELISA:
A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.
Western blot:
1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).
Immunohistochemistry:
10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.
Optimal working dilutions must be determined by end user.
10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).
Immunohistochemistry:
10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.
ELISA:
A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.
Western blot:
1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).
Immunohistochemistry:
10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.
Optimal working dilutions must be determined by end user.
Control:
HeLa whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.
Presentation:
Protein G Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ACTA1
- MPFD
- ASMA
- NEM1
- CFTDM
- ACTA
- CFTD
- NEM2
- Alpha-actin-1
- CFTD1
- NEM3
Alternate Names:
- actin, alpha 1, skeletal muscle
- alpha skeletal muscle actin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Key Applications:
- ELISA
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Brand Family:
Chemicon
Product Name:
Anti-Actin, Clone C4
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Protein G Purified
Host:
Mouse