Ordering Information
Anti-Bim, clone 14A8
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IH, IP, WB | Rat | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P09211 # Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
SIZE: 210 amino acids; 23356 Da
SUBUNIT: Homodimer.
SIMILARITY: SwissProt: P09211 ## Belongs to the GST superfamily. Pi family.
SIZE: 210 amino acids; 23356 Da
SUBUNIT: Homodimer.
SIMILARITY: SwissProt: P09211 ## Belongs to the GST superfamily. Pi family.
Entrez Gene Number:
Entrez Gene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are th
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Description:
Anti-Bim, clone 14A8
Clone:
14A8
Specificity:
Purified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
The antibody is intended for the detection of all splice variants of Bim in a range of techniques including:
Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.
immunoprecipitation
FACS
immunohistochemistry.
It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.
Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.
immunoprecipitation
FACS
immunohistochemistry.
It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.
Species Reactivity:
- Human
- Mouse
Isotype:
IgG2a
Immunogen:
Recombinant mouse BimShort (BimS) protein.
Molecular Weight:
~17-23kDa
Control:
Skin/Skin Tumor
Presentation:
Protein A Purified mouse immunoglobulin in PBS containing 0.05% Bronidox as preservative.
Storage Conditions:
Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- GSTP1
- DFN7
- GST3
- GSTP1-1
- FAEES3
- PI
- EC 2.5.1.18
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
BCL2 & Inhibition
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Rat
Product Overview:
Purity: The antibody is shown to be >95% pure as determined by SDS-PAGE. The BimS antibody is supplied 0.22 micron filtered.
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein A Purified