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Anti-Bim, clone 14A8
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IH, IP, WB | Rat | Purified | Monoclonal Antibody |
Description:
Anti-Bim, clone 14A8
Trade Name:
Chemicon (Millipore)
Product Overview:
Purity: The antibody is shown to be >95% pure as determined by SDS-PAGE. The BimS antibody is supplied 0.22 micron filtered.
Specificity:
Purified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.
Molecular Weight:
~17-23kDa
Immunogen:
Recombinant mouse BimShort (BimS) protein.
Clone:
14A8
Isotype:
IgG2a
Background Information:
Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
Species Reactivity:
- Human
- Mouse
Application Notes:
The antibody is intended for the detection of all splice variants of Bim in a range of techniques including:
Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.
immunoprecipitation
FACS
immunohistochemistry.
It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.
Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.
immunoprecipitation
FACS
immunohistochemistry.
It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.
Control:
Skin/Skin Tumor
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in PBS containing 0.05% Bronidox as preservative.
Storage Conditions:
Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
UniProt Summary:
Function: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
Isoform BimEL: Mitochondrion
Isoform BimL: Mitochondrion
Isoform BimS: Mitochondrion
Isoform Bim-alpha1: Mitochondrion
Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities: Belongs to the Bcl-2 family.
Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
Isoform BimEL: Mitochondrion
Isoform BimL: Mitochondrion
Isoform BimS: Mitochondrion
Isoform Bim-alpha1: Mitochondrion
Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities: Belongs to the Bcl-2 family.
Qty/Pk:
50 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Rat
Product Name:
Anti-Bim, clone 14A8
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody