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Immunoprecipitation:
Representative lot data.
Immunoprecipitated and blotted with rabbit anti-ATG5 antibody (AB15404)
Arrow indi...
Western Blot Analysis:
Representative lot data.
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-A...
Anti-ATG5, clone 177.19
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-ATG5, clone 177.19
Specificity:
The antibody reacts with the 50 kDa ATG5 protein.
Molecular Weight:
50 kDa
Immunogen:
Linear Peptide, aa: 8-318
Clone:
177.19
Isotype:
IgG2bκ
Background Information:
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human. Other species have not been tested.
Application Notes:
Immunoprecipitation: HeLa cell lysate
Control:
Western Blot and Immunoprecipitation: HeLa cell lysate.
Quality Assurance:
Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- APG5
- APG5-LIKE
- APG5L
- ASP
- hAPG5
Alternate Names:
- APG5 autophagy 5-like
- APG5 autophagy 5-like (S. cerevisiae)
- APG5 (autophagy 5, S. cerevisiae)-like
- ATG5 autophagy related 5 homolog (S. cerevisiae)
- ATG5 autophagy related 5-like
- Apoptosis-specific protein
- apoptosis specific protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
UniProt Summary:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed By similarity.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
SUBCELLULAR LOCATION: Cytoplasm. Note= Colocalizes with nonmuscle actin.
TISSUE SPECIFICTY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
INDUCTION: By apoptotic stimuli.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SEQUENCE SIMILARITIES: Belongs to the ATG5 family
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
SUBCELLULAR LOCATION: Cytoplasm. Note= Colocalizes with nonmuscle actin.
TISSUE SPECIFICTY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
INDUCTION: By apoptotic stimuli.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SEQUENCE SIMILARITIES: Belongs to the ATG5 family
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Product Name:
Anti-ATG5, clone 177.19
Concentration:
1.0 mg/ml
Antibody Type:
Monoclonal Antibody