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Ordering Information
- : MAB318
- : 100 µL
- : Variable
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Mouse anti-Tyrosine Hydroxylase (Catalog Number MAB318) Staining of rat dorsal root ganglia (DRG) after sciatic nerve transection for 2 weeks. Stainin...
Mouse anti-Tyrosine Hydroxylase (Catalog Number MAB318) staining of embryonic rat mesencephalic dopaminergic neurons
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Anti-Tyrosine Hydroxylase, clone LNC1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Fg, H, M, Mk, R, Lz, Vo, Zebrafish | IH, IH(P), IP, WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Tyrosine Hydroxylase, clone LNC1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
Molecular Weight:
59-63 kDa
Immunogen:
Tyrosine Hydroxylase purified from PC12 cells
Clone:
LNC1
Isotype:
IgG1κ
Background Information:
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Species Reactivity:
- Chicken
- Frog
- Human
- Mouse
- Monkey
- Rat
- Lizard
- Vole
- Zebrafish
Species Reactivity Note:
Human, mouse, rat, chicken, frog, lizard, monkey, vole and zebrafish.
Application Notes:
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
Control:
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Purification Method:
Unpurified
Presentation:
Ascites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TH
- TYH
- EC 1.14.16.2
Alternate Names:
- TH
- Tyrosine Monooxygenase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
UniProt Summary:
FUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Brand Family:
Chemicon
Product Name:
Anti-Tyrosine Hydroxylase, clone LNC1
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse