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Anti-Poly ADP-ribose, clone 10H
Recommended Replacement for: MAB3191| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | IC, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Poly ADP-ribose, clone 10H
Replaces:
MAB3191
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes poly (ADP-ribose)-modified proteins. No cross-reaction with RNA, DNA, monomers of ADP-ribose or NAD.
Immunogen:
Purified poly (ADP-ribose) polymer, chain length of 2-50 units.
Clone:
10H
Isotype:
IgG3a
Species Reactivity:
All
Application Notes:
Western blot: 1:1000
Immunoprecipitation
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunoprecipitation
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Control:
poly (ADP-ribose)-modified proteins.
Purification Method:
Protein A purfied
Presentation:
Purified immunoglobulin in PBS containing 0.1% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Qty/Pk:
50 μL
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Protein/Isoform Description:
Poly(ADP-Ribose) Polymerase 1 (PARP-1), when activated by breaks in DNA, poly ADP-ribosylates nuclear proteins, resulting in NAD+ depletion and cell death by apoptosis. During apoptosis, Caspase 3 cleaves PARP into signature 85 and 24 kDa fragments. The 24 kDa fragment irreversibly binds to the broken ends of DNA, preventing access of repair enzymes to DNA, and ensuring the irreversibility of apoptosis. PARP cleavage is commonly used as a marker to "prove" cell death by apoptosis versus necrosis. Western blot detection of PARP cleavage (appearance of the 85 kDa fragment) is widely used as a readout of apoptosis.
Product Name:
Anti-Poly ADP-ribose, clone 10H
Concentration:
Variable
Antibody Type:
Monoclonal Antibody