Product Images
Rabbit anti-MAP2 (Catalogue No. AB5622). Immunolocalization of MAP2 (green) and GFAP (red) in rat hippocampus. The slide was mounted with Vectashield ...
Mouse anti-GFAP (Catalog Number MAB360). Immunolocalization of GFAP (red) and MAP2 (blue) in rat hippocampus. Photo courtesy of Karl A. Kasischke & Pa...
see more >. Immunolocalization of MAP2 (green) and GFAP (red) in rat hippocampus. The slide was mounted with Vectashield DAPI. Photo courtesy of Karl A. Kasischke & Patricia J. Fisher, Cornell University, Ithaca, NY.'))
. Immunolocalization of GFAP (red) and MAP2 (blue) in rat hippocampus. Photo courtesy of Karl A. Kasischke & Patricia J. Fisher, Cornell University, Ithaca, NY.'))
: Representative images from a previous lot.<br />Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain'))
)
. <br />Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.<br /><br />Arrow indicates protein GFAP (~51 kDa).'))
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB360
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, Po, R, Rb | ICC, IHC, IH(P), WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB360
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Molecular Weight:
~ 51 kDa
Immunogen:
Purified GFAP from porcine spinal cord.
Clone:
GA5
Isotype:
IgG1
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Pig
- Rat
- Rabbit
Species Reactivity Note:
Reacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined.
Application Notes:
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Control:
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Quality Assurance:
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Purification Method:
Unpurified
Presentation:
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GFAP
- FLJ45472
Alternate Names:
GFAP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Entrez Gene Summary:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
UniProt Summary:
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
Brand Family:
Chemicon
Product Name:
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB360
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse