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Ordering Information
- : MAB4146
- : 100 μg
- : 0.25 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
HepG2 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with a...
Anti-BCRP, clone BXP-21
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, IH, IH(P), WB | Mouse | Culture Supernatant | Monoclonal Antibody |
Description:
Anti-BCRP, clone BXP-21
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.
Molecular Weight:
70 kDa
Immunogen:
Fusion protein consisting of the E. coli maltose-binding protein and a 126-aa portion of BCRP (aa 271-396).
Clone:
BXP-21
Isotype:
IgG2a
Background Information:
The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates
Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Immunocytochemistry:
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.
Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.
Optimal working dilutions must be determined by end user.
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.
Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.
Optimal working dilutions must be determined by end user.
Control:
MCF7 cells
Quality Assurance:
Evaluated by Western Blot on HepG2 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.
Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.
Purification Method:
Unpurified
Presentation:
Mouse monoclonal IgG2a in buffer containing 0.7% BSA and 0.1% sodium azide
Storage Conditions:
For long term storage, store at -20ºC. Stable for 6 months from date of receipt at 2-8ºC.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ABCG2
- BCRP
- BMDP
- BCRP1
- MXR
- ABC15
- ABCP
- EST157481
- MGC102821
- MXR1
- MRX
- CDw338
Alternate Names:
- Breast Cancer Resistance Protein
- Placenta-specific ATP Binding Cassette Transporter
- ATP-binding Cassette, Sub Family G, Member 2 (ABCG2)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Entrez Gene Summary:
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
UniProt Summary:
FUNCTION: SwissProt: Q9UNQ0 # Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123.
SIZE: 655 amino acids; 72314 Da
SUBUNIT: Monomer or homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in placenta. Low expression in small intestine, liver and colon.
PTM: Glycosylation-deficient ABCG2 is normally expressed and functional.
SIMILARITY: SwissProt: Q9UNQ0 ## Belongs to the ABC transporter family. ABCG (White) subfamily. & Contains 1 ABC transporter domain.
SIZE: 655 amino acids; 72314 Da
SUBUNIT: Monomer or homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in placenta. Low expression in small intestine, liver and colon.
PTM: Glycosylation-deficient ABCG2 is normally expressed and functional.
SIMILARITY: SwissProt: Q9UNQ0 ## Belongs to the ABC transporter family. ABCG (White) subfamily. & Contains 1 ABC transporter domain.
Stem Cell Type:
- Neural Stem Cells
- Hematopoietic Stem Cells
Stem Cell Workflow Stage:
Characterization
Brand Family:
Chemicon
Product Name:
Anti-BCRP, clone BXP-21
Concentration:
0.25 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Culture Supernatant
Host:
Mouse