Ordering Information
Product Images
P-glycoprotein expression on human melanoma cells as detected by Mab MM12.10 in confocal microscopy studies.
Anti-MDR1, extracellular human specific Pgp, clone MM12.10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC ... see more »
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC ... see more »
Entrez Gene Number:
Entrez Gene Summary
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux
...
see more »
Description:
Anti-MDR1, extracellular human specific Pgp, clone MM12.10
Alternate Names:
P-glycoprotein; CD243; p170; Pgp
Clone:
MM12.10
Specificity:
MDR-1 clone MM12.10 recognizes a mapped epitope in the extracellular Pgp domain and detects low-level Pgp expression in living (intact) human MDR cells (Cianfriglia, 2002). The epitope has been mapped to the single amino acid level using different techniques (Romagnoli, 1999).
Key Applications:
- Flow Cytometry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunohistochemistry on formalin fixed paraffin embedded sections
Confocal and Electron Microscopy
ELISA
Functional Studies (Cianfriglia, 2002)
Optimal working dilutions must be determined by the end user.
Confocal and Electron Microscopy
ELISA
Functional Studies (Cianfriglia, 2002)
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Isotype:
IgG2aκ
Immunogen:
Living intact human multidrug-resistant cells
Epitope:
extracellular human specific Pgp
Presentation:
Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Gene Symbol:
- ABCB1
- PGY1
- MGC163296
- GP170
- ABC20
- CD243
- P-gp
- MDR1
- CLCS
- EC 3.6.3.44
Antibody Category:
Metabolism
Antibody Sub-Category:
Toxicology & Drug Resistance
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody