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注文に関する情報
- : MAB4161
- : 100 µg
- : 1 mg/mL
画像情報
P-glycoprotein expression on human melanoma cells as detected by Mab MM12.10 in confocal microscopy studies.
Anti-MDR1, extracellular human specific Pgp, clone MM12.10
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H | FC, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-MDR1, extracellular human specific Pgp, clone MM12.10
Trade Name:
Chemicon (Millipore)
Specificity:
MDR-1 clone MM12.10 recognizes a mapped epitope in the extracellular Pgp domain and detects low-level Pgp expression in living (intact) human MDR cells (Cianfriglia, 2002). The epitope has been mapped to the single amino acid level using different techniques (Romagnoli, 1999).
Epitope:
extracellular human specific Pgp
Immunogen:
Living intact human multidrug-resistant cells
Clone:
MM12.10
Isotype:
IgG2aκ
Species Reactivity:
Human
Application Notes:
Immunohistochemistry on formalin fixed paraffin embedded sections
Confocal and Electron Microscopy
ELISA
Functional Studies (Cianfriglia, 2002)
Optimal working dilutions must be determined by the end user.
Confocal and Electron Microscopy
ELISA
Functional Studies (Cianfriglia, 2002)
Optimal working dilutions must be determined by the end user.
Presentation:
Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ABCB1
- PGY1
- MGC163296
- GP170
- ABC20
- CD243
- P-gp
- MDR1
- CLCS
- EC 3.6.3.44
Alternate Names:
P-glycoprotein; CD243; p170; Pgp
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Key Applications:
- Flow Cytometry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
SIZE: 1280 amino acids; 141463 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-MDR1, extracellular human specific Pgp, clone MM12.10
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody