Anti-Cathepsin D, clone C5

P07339

FUNCTION: SwissProt: P07339 # Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
SIZE: 412 amino acids; 44552 Da
SUBUNIT: Consists of a light chain and a heavy chain.
SUBCELLULAR LOCATION: Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
DISEASE: SwissProt: P07339 # Defects in CTSD are the cause of neuronal ceroid lipofuscinosis 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. The neuronal ceroid lipofuscinosis are a group of progressive neurodegenerative diseases in children and in adults, characterized by visual and mental decline, motor disturbance, epilepsy and behavioral changes.
SIMILARITY: SwissProt: P07339 ## Belongs to the peptidase A1 family. ... hide » see more »

NM_001909.3

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. ... hide » see more »

Anti-Cathepsin D, clone C5

Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.

C5

Human Cathepsin-D. Fine granular cytoplasmic staining is seen in macrophages and tumor cells reflecting the lysosomal location of the protein. Thus stained cells will often be inconspicuous at low power when only a light staining effect may be seen; medium and high power reveal the lysosomal staining.

Immunohistochemistry (Paraffin)

Immunohistochemistry

Typical working dilution 1:100-1:200 using standard ABC technique, 60 minutes incubation at 25°C.

Effective on paraffin-embedded and fresh frozen tissue. Trypsinisation may be required.

Human

IgG2b

Cathespin-D purified from human spleen.

POSITIVE CONTROL:

Many tissues, e.g. most breast carcinomas, have a built-in control in the form of tissue macrophages. Alternatively human liver can be used as a control.

Liquid tissue culture supernatant containing 15 mM sodium azide.

Maintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

• CTSD
• CLN10
• MGC2311
• CPSD
• EC 3.4.23.5 [Contains: Cathepsin D light chain
• Cathepsin D heavy chain].

• Apoptosis & Cancer
• Metabolism

• Apoptosis - Additional
• Enzymes & Biochemistry

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Chemicon (Millipore)

Culture Supernatant

Mouse

Monoclonal Antibody