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  • : 500 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
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Negative control staining of Human Tonsil, tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, IHC-Select Detection with HRP-DAB...

Negative control staining of Human Tonsil, tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, IHC-Select Detection with HRP-DAB...

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Anti-PCNA Antibody, clone PC10

Species Reactivity Key Applications Host Format Antibody Type
Inv, Vrt ELISA, FC, IH(P), IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-PCNA Antibody, clone PC10
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.
Molecular Weight:
36 kDa
Immunogen:
Rat PCNA made in the protein A vector pR1T2T.
Clone:
Isotype:
IgG2a
Background Information:
Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.
Species Reactivity:
  • Invertebrates
  • Vertebrates
Application Notes:
Western blot: 1:250-1:1000. Nuclear extracts are preferred over whole cell protein extracts.

Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.

Immunoprecipitation

Indirect Flow Cytometry

Optimal working dilutions must be determined by the end user.
Control:
Rat kidney, Human tonsil, lymph node tissue
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in PBS with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • PCNA
  • Cyclin
  • cyclin
  • MGC8367
Alternate Names:
Proliferating Cell Nuclear Antigen; DNA Polymerase delta Processivity Factor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
UniProt Summary:
FUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
SIZE: 261 amino acids; 28769 Da
SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
SUBCELLULAR LOCATION: Nucleus.
PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
Brand Family:
Chemicon
Product Name:
Anti-PCNA Antibody, clone PC10
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
500 µg
Format:
Purified
Host:
Mouse
Applications:
Use Anti-PCNA Antibody, clone PC10 (mouse monoclonal antibody) validated in ELISA, FC,IHC(P), IP, WB to detect PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor.

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